MHC Class II Antibody (A6-61.3) - BSA Free
Novus Biologicals | Catalog # NBP3-45120
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Mouse
Applications
Radioimmunoassay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2a Kappa Clone # A6-61.3
Format
BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a fusion of the non-producing plasmacytoma Ag8.653 with spleen cells from ATL mice that had been immunized with ATH spleen cells.
Specificity
This antibody is specific for mouse I-A, haplotypes H-2k, d and q. It binds to the beta-chain of mouse Ia.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for MHC Class II Antibody (A6-61.3) - BSA Free
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] -
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on coverslips stained with NBP3-45120 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-45120, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] -
Flow Cytometry: MHC Class II Antibody (A6-61.3) - BSA Free [NBP3-45120] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody ( isotype control, black line) or NBP3-45120 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for MHC Class II Antibody (A6-61.3) - BSA Free
Application
Recommended Usage
Radioimmunoassay
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MHC Class II
Alternate Names
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbol
HLA-DMA
Additional MHC Class II Products
Product Documents for MHC Class II Antibody (A6-61.3) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MHC Class II Antibody (A6-61.3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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