MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free

Name: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-25675
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-25675

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1888 expressed in HEK293

Format

Azide and BSA Free

View all MKK4 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MKK4/MEK4. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] -

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] -

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Untransfected (-) and transfected (+) HeLa whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] -

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] -

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (NBP3-25675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free [NBP3-25675] -

View 3 more images

Applications for MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MKK4/MEK4 Antibody (HL1888) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.