MLKL Antibody (SA40-04)

Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]

Western Blot: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of MLKL on different cell lysates using anti-MLKL antibody at 1/1,000 dilution. Positive control: Lane 1: HT29 Lane 2: HUVEC Lane 3: Hela

Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299]

Immunohistochemistry-Paraffin: MLKL Antibody (SA40-04) [NBP2-67299] - Analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. Counter stained with hematoxylin.

Western Blot: MLKL Antibody (SA40-04) [NBP2-67299]

Western blot analysis on different lysates with NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane) Lane 2: HT-29 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: U-937 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (low expression) (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67299 at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.