MMP-11 Antibody (SN74-08)

Novus Biologicals | Catalog # NBP2-67670

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN74-08 expressed in HEK293
View all MMP-11 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human MMP-11 aa 439-488 / 488. (SwissProt: P24347 Human; SwissProt: Q02853 Mouse; SwissProt: Q499S5 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-11 Antibody (SN74-08)

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of MMP11 on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded mouse spleen tissue using anti-MMP11 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/ Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunocytochemistry/Immunofluorescence: MMP-11 Antibody (SN74-08) [NBP2-67670] - Staining MMP11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP11 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670]

Immunohistochemistry-Paraffin: MMP-11 Antibody (SN74-08) [NBP2-67670] - Analysis of paraffin-embedded human spleen tissue using anti-MMP11 antibody. Counter stained with hematoxylin.
MMP-11 Antibody (SN74-08) Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western blot analysis on different lysates with NBP2-67670 at 1/2,000 dilution. Lane 1: 293T cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (20 µg/Lane) Lane 6: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67670 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
MMP-11 Antibody (SN74-08) Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western Blot: MMP-11 Antibody (SN74-08) [NBP2-67670]

Western blot analysis on different lysates with NBP2-67670 at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A549 cell lysate Lane 1: Neuro-2a cell lysate Lane 1: NIH/3T3 cell lysate Lane 1: RAW264.7 cell lysate Lane 1: L-929 cell lysate Lane 1: PC-12 cell lysate Lane 1: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67670 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for MMP-11 Antibody (SN74-08)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-11

The matrix metalloproteinases are a tightly regulated family of enzymes that degrade extracellular matrix and basement membrane components. Recent evidence suggests that these proteases and their specific inhibitors play important roles in normal developmental processes and in pathological conditions (1). MMP-11 (stromelysin-3), a new member of the matrix metalloproteinase family, is expressed in tissues undergoing the active remodeling associated with embryonic development, wound healing and tumor invasion. But like all other members of the matrix metalloproteinase gene family, stromelysin-3 is synthesized as an inactive precursor that must be processed to its mature form in order to express enzymic activity (2). MMP-11, like an inflammatory mediator, may exert a chemotactic influence on macrophages which aggregate in the mesangium; MMP-11 is not likely to have a parallel mitogenic or antifibrotic effect in diseased glomeruli (3).

Long Name

Matrix Metalloproteinase 11

Alternate Names

MMP11

Gene Symbol

MMP11

Additional MMP-11 Products

Product Documents for MMP-11 Antibody (SN74-08)

Certificate of Analysis

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Product Specific Notices for MMP-11 Antibody (SN74-08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

Citations for MMP-11 Antibody (SN74-08)

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Protocols

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