Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.
Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
R&D Systems | Catalog # AF4960
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Angiopoietin-like Protein 7/ANGPTL7
Gln22-Pro337
Accession # Q8R1Q3
Gln22-Pro337
Accession # Q8R1Q3
Specificity
Detects mouse Angiopoietin-like Protein 7/ANGPTL7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human ANGPTL7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Detection of Rat Angiopoietin-like Protein 7/ANGPTL7 by Western Blot.
Western blot shows lysates of rat eye tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Angiopoietin-like Protein 7/ANGPTL7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-like Protein 7/ANGPTL7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat eye tissue
Sample: Rat eye tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Angiopoietin-like Protein 7/ANGPTL7
Long Name
Angiopoietin-like Protein 7
Alternate Names
ANGPTL7, AngX, CDT6
Entrez Gene IDs
Gene Symbol
ANGPTL7
UniProt
Additional Angiopoietin-like Protein 7/ANGPTL7 Products
- All Products for Angiopoietin-like Protein 7/ANGPTL7
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Product Documents for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse Angiopoietin-like Protein 7/ANGPTL7 Antibody
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Q: This product says it can only be used with mouse so why does the picture say it was used in rat?
A: AF4960 is manufactured by our sister company, R&D Systems. The antibody was tested against rat lysate by Western blotting, however rat has not been listed as a validated species since a full range of tests with rat samples have not yet been performed.
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