Mouse Bax Antibody (1127504)

R&D Systems | Catalog # MAB11842

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1127504
View all Bax Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide Accession # Q07813

Specificity

Detects a synthetic peptide specific for Mouse BAX around amino acid 5 in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Bax Antibody (1127504)

Detection of Mouse Bax by Western Blot.

Western Blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse Bax Monoclonal Antibody (Catalog # MAB11842) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Bax at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse Bax Antibody (1127504)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse spleen tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bax

BAX (BCL2-associated X protein) is a pro-apoptotic member of the BCL-2 protein family, with a molecular weight of approximately 21 kDa. BAX plays a critical role in regulating mitochondrial-mediated apoptosis, a form of programmed cell death essential for maintaining cellular homeostasis and responding to stress or damage. Upon activation, BAX translocates to the mitochondrial outer membrane, where it oligomerizes to form pores that facilitate the release of cytochrome c and other apoptotic factors into the cytosol, triggering caspase activation and cell death. The expression and function of BAX are tightly regulated, as dysregulation can contribute to pathological conditions such as cancer, neurodegenerative diseases, and immune disorders. Mutations or altered expression of BAX have been linked to therapeutic resistance in cancer, underscoring its role as a pivotal player in cell death mechanisms and a potential biomarker for chemotherapy responsiveness. Furthermore, BAX's interactions with other BCL-2 family proteins, such as BAK and anti-apoptotic members like BCL-2 and BCL-XL, highlight its importance in coordinating cellular responses to apoptotic stimuli.

References

  1. Chao DT, Korsmeyer SJ. BCL-2 family: regulators of cell death. Annu Rev Immunol. 1998;16:395-419. doi: 10.1146/annurev.immunol.16.1.395. PMID: 9597135.
  2. Gavathiotis E, Reyna DE, Davis ML, Bird GH, Walensky LD. BH3-triggered structural reorganization drives the activation of proapoptotic BAX. Mol Cell. 2010 Nov 12;40(3):481-92. doi: 10.1016/j.molcel.2010.10.019. PMID: 21070973; PMCID: PMC3050027.
  3. Maes ME, Grosser JA, Fehrman RL, Schlamp CL, Nickells RW. Completion of BAX recruitment correlates with mitochondrial fission during apoptosis. Sci Rep. 2019 Nov 12;9(1):16565.

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

Additional Bax Products

Product Documents for Mouse Bax Antibody (1127504)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Bax Antibody (1127504)

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

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