Mouse Bcl-2 Minus C-Terminus Antibody (121529)

R&D Systems | Catalog # MAB810

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 121529
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Bcl-2

Specificity

Detects mouse Bcl‑2 Minus C-Terminus in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Bcl-2 Minus C-Terminus Antibody (121529)

Detection of Mouse Bcl‑2 antibody by Western Blot.

Detection of Mouse Bcl‑2 by Western Blot.

Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Rat Bcl-2 by Simple Western

Detection of Rat Bcl-2 by Simple Western

TUNEL assay of cardiac myocyte apoptosis in the hearts. (A) Quantification of TUNEL-positive nuclei n=3. Representative qPCR (B) and western blot (C) analysis (original blots/gels are presented in Supplementary 2 Fig.7-9) of apoptosis-related mRNA and protein, n=3, Chemiluminescence signals were visualized and cropped using Protein Simple Compass software. The whole cell lysates were used for western blot. The data are presented as the mean SEM; *P<0.05; **P<0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/40188200/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Mouse Bcl-2 Minus C-Terminus Antibody (121529)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: CTLL-2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-2

The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bcl-2 is a 26 kD protein associated with cell survival. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis in a number of cells. The ratio of Bcl-2 to other Bcl-2 family members and subcellular localization are believed to modulate the apoptotic process.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Entrez Gene IDs

596 (Human); 12043 (Mouse); 24224 (Rat)

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Mouse Bcl-2 Minus C-Terminus Antibody (121529)

Certificate of Analysis

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Product Specific Notices for Mouse Bcl-2 Minus C-Terminus Antibody (121529)

For research use only

Citations for Mouse Bcl-2 Minus C-Terminus Antibody (121529)

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