Mouse CCL11/Eotaxin Antibody [Biotin]

R&D Systems | Catalog # BAF420

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL11/Eotaxin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL11/Eotaxin (R&D Systems, Catalog # 420-ME)
His24-Pro97
Accession # P48298

Specificity

Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse CCL24/Eotaxin-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CCL11/Eotaxin Antibody [Biotin]

CCL11/Eotaxin antibody in Mouse Colon by Immunohistochemistry (IHC-Fr).

CCL11/Eotaxin in Mouse Colon.

CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Mouse CCL11 / Eotaxin ELISA Standard Curve

Mouse CCL11 / Eotaxin ELISA Standard Curve

Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME) was serially diluted and captured by Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL11/Eotaxin Antibody [Biotin]

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse colon

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)

Mouse CCL11/Eotaxin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CCL11/Eotaxin Antibody (Catalog # AF-420-NA)
  • Standard: Recombinant Mouse CCL11/Eotaxin Protein (Catalog # 420-ME)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using BAF420 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

References

  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

Alternate Names

Eotaxin

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Gene Symbol

CCL11

UniProt

P48298

Additional CCL11/Eotaxin Products

Product Documents for Mouse CCL11/Eotaxin Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL11/Eotaxin Antibody [Biotin]

For research use only

Citations for Mouse CCL11/Eotaxin Antibody [Biotin]

Customer Reviews for Mouse CCL11/Eotaxin Antibody [Biotin] (2)

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  • Mouse CCL11/Eotaxin Biotinylated Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/29/2022
    Used as detection in ELISA
    Mouse CCL11/Eotaxin Antibody [Biotin] BAF420
  • Mouse CCL11/Eotaxin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 08/07/2019
    Mouse CCL11/Eotaxin Antibody [Biotin] BAF420

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