Mouse CCL2/JE/MCP-1 Antibody

R&D Systems | Catalog # AB-479-NA

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Cell Culture, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # Q5SVU3

Specificity

Detects mouse CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human MCP-1, recombinant mouse (rm MCP-5, and recombinant rat JE is observed. In Western blots, no cross-reactivity with rmEotaxin, rmMCP-5, or rmCCL7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL2/JE/MCP-1 Antibody

Detection of Recombinant Mouse CCL2/JE/MCP-1 antibody by Western Blot.

Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot.

Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutral-ization by Mouse CCL2/JE Antibody.

Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutral-ization by Mouse CCL2/JE Antibody.

Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND50 is typically 10-30 µg/mL.

Applications for Mouse CCL2/JE/MCP-1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)

Neutralization

Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

References

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

Q5SVU3

Additional CCL2/JE/MCP-1 Products

Product Documents for Mouse CCL2/JE/MCP-1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL2/JE/MCP-1 Antibody

For research use only

Citations for Mouse CCL2/JE/MCP-1 Antibody

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