Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
Mouse CCL2/JE/MCP-1 Antibody [Biotin]
R&D Systems | Catalog # BAF479
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)
Gln24-Arg96
Accession # Q5SVU3
Gln24-Arg96
Accession # Q5SVU3
Specificity
Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse CCL2/JE/MCP-1 Antibody [Biotin]
Mouse CCL2 / JE / MCP-1 ELISA Standard Curve
Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL2/JE/MCP-1 Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)
Sample: Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)
Mouse CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using BAF479 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL2/JE/MCP-1
References
- Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
- Gu, L. et al. (1999) Chem. Immunol. 72:7.
- Luini, W. et al. (1994) Cytokine 6:28.
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
UniProt
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP-1 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL2/JE/MCP-1 Antibody [Biotin]
For research use only
Citations for Mouse CCL2/JE/MCP-1 Antibody [Biotin]
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Application: Immunohistochemistry-FrozenSample Tested: See PMID: 23410977Species: MouseVerified Customer | Posted 01/15/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
