CCL22, also known as ABCD-1 and MDC (macrophage-derived chemokine), is a CC chemokine cloned from activated mouse B cells. Mouse CCL22 cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human CCL22 share 64% identity and 83% similarity. The genomic organization of the mouse and human CCL22 genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse CCL22 mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. CCL22 is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.
Mouse CCL22/MDC Antibody (158132)
R&D Systems | Catalog # MAB4391
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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, ELISA Development, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 158132
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # O88430
Gly25-Ser92
Accession # O88430
Specificity
Detects mouse CCL22/MDC in ELISAs and Western blots. In Western blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL22/MDC Antibody (158132)
Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody.
Mouse CCL22/MDC Antibody (Catalog # MAB4391) neutralizes CCL22/MDC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 1.00 - 12.0 μg/mL in the presence of 10.0 ng/mL Recombinant Mouse CCL22/MDC.
Mouse CCL22 / MDC ELISA Standard Curve
Recombinant Mouse CCL22/MDC (Catalog # 439-MD) was serially diluted and captured by Rat Anti-Mouse CCL22/MDC Monoclonal Antibody (Catalog # MAB4391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL22/MDC Antibody (158132)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 1.00 - 12.0 μg/mL in the presence of 10.0 ng/mL Recombinant Mouse CCL22/MDC.
Mouse CCL22/MDC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4391 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL22/MDC
References
- Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
- Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
- Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
- Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
Alternate Names
ABCD-1, MDC, STCP-1
Gene Symbol
CCL22
UniProt
Additional CCL22/MDC Products
Product Documents for Mouse CCL22/MDC Antibody (158132)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL22/MDC Antibody (158132)
For research use only
Citations for Mouse CCL22/MDC Antibody (158132)
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Application: ELISASample Tested: SerumSpecies: MouseVerified Customer | Posted 05/13/2021This antibody was used to coat the wells of a 96-well plate in conjunctions with BAF439 in an immunoassay for MDC.
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