Mouse CCL25/TECK Antibody [Biotin]

R&D Systems | Catalog # BAF481

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry-Frozen, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL25/TECK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL25/TECK (R&D Systems, Catalog # 481-TK)
Gln24-Asn144
Accession # O35903

Specificity

Detects mouse CCL25/TECK in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human CCL25/TECK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CCL25/TECK Antibody [Biotin]

Mouse CCL25 / TECK ELISA Standard Curve

Mouse CCL25 / TECK ELISA Standard Curve

Recombinant Mouse CCL25/TECK (Catalog # 481-TK) was serially diluted and captured by Rat Anti-Mouse CCL25/TECK Monoclonal Antibody (Catalog # MAB4811) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL25/TECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-481-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL25/TECK Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL25/TECK (Catalog # 481-TK)

Mouse CCL25/TECK Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CCL25/TECK Antibody (Catalog # MAB4811)
  • Standard: Recombinant Mouse CCL25/TECK Protein (Catalog # 481-TK)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using BAF481 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Human CCL25 cDNA has also been cloned and shown to encode a 151 amino acid protein that exhibits 49% amino acid sequence identity to mouse CCL25. The expression of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered.

References

  1. Vicari, A.P. et al. (1997) Immunity 7:291.

Alternate Names

TECK

Entrez Gene IDs

6370 (Human); 20300 (Mouse)

Gene Symbol

CCL25

UniProt

O35903

Additional CCL25/TECK Products

Product Documents for Mouse CCL25/TECK Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL25/TECK Antibody [Biotin]

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL25/TECK Antibody [Biotin]

Customer Reviews for Mouse CCL25/TECK Antibody [Biotin] (1)

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  • Mouse CCL25/TECK Biotinylated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Thymus tissue
    Species: Mouse
    Verified Customer | Posted 02/13/2023
    I tested the antibody for Flow Cytometry - but it didn't go as wished. There is no clear difference between the positive and negative population.
    Mouse CCL25/TECK Antibody [Biotin] BAF481
    Bio-Techne Response
    Product used in an unvalidated application.

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