Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CD200 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD200
Gln31-Gly232
Accession # O54901

Specificity

Detects mouse CD200 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human CD200 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD200 Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD)

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200

CD200, also known as OX-2, is a 45 kDa type I transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The mouse CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-likeV-type and one Ig-like C2-type domain (3). Splice variants of CD200 have been described in human but not in mouse. Within the ECD, mouse CD200 shares 76% and 94% aa sequence identity with human and rat CD200, respectively.

References

    Alternate Names

    CD200, MOX1, MOX2, MRC, OX-2

    Entrez Gene IDs

    4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

    Gene Symbol

    CD200

    UniProt

    O54901 (Goat)

    Additional CD200 Products

    Product Documents for Mouse CD200 Antibody [Biotin]

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Mouse CD200 Antibody [Biotin]

    For research use only

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