CD229, also known as T lymphocyte surface antigen Ly-9, is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is also a member of the CD150/SLAM receptor family and is expressed on T and B lymphocytes. Mouse CD229 contains 2 Ig-like C2-type domains and 2 Ig-like V-type domains in its extracellular region. Two mouse alleles that differ in 6 extracellular amino acid residues have been reported. Human and mouse CD229 share 60% amino acid sequence identity in their extracellular regions.
Mouse CD229/SLAMF3 Antibody (312830)
R&D Systems | Catalog # MAB2555
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 312830
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD229/SLAMF3
Lys48-Phe454
Accession # AAH95921
Lys48-Phe454
Accession # AAH95921
Specificity
Detects mouse CD229/SLAMF3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CD229 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD229/SLAMF3 Antibody (312830)
Detection of CD229/SLAMF3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD229/SLAMF3 Monoclonal Antibody (Catalog # MAB2555, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).Applications for Mouse CD229/SLAMF3 Antibody (312830)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Western Blot
1 µg/mL
Sample: Recombinant Mouse CD229/SLAMF3 under non-reducing conditions only
Sample: Recombinant Mouse CD229/SLAMF3 under non-reducing conditions only
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CD229/SLAMF3
Long Name
SLAM Family Member 3
Alternate Names
CD229, Ly9, SLAMF3
Gene Symbol
LY9
UniProt
Additional CD229/SLAMF3 Products
Product Documents for Mouse CD229/SLAMF3 Antibody (312830)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CD229/SLAMF3 Antibody (312830)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Conditions
- Western Blot Protocol
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
