Mouse Cerberus 1 Antibody (225807)

R&D Systems | Catalog # MAB1986

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 225807
View all Cerberus 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
Asp18-Pro272
Accession # O55233

Specificity

Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Cerberus, recombinant mouse (rm) DAN, or rmGremlin.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Cerberus 1 Antibody (225807)

Detection of Cerberus 1 by Immunohistochemistry

Detection of Cerberus 1 by Immunohistochemistry

The molecular signature of the primitive streak cells contributing to the ventricles or outflow/atria are distinct.(A) Mid-late streak and OB/EB TnGPF-CreERT2/+; R26RtdTomato/tdTomato embryos analysed in the scRNA-seq. Embryos were selected at the mid-late streak stages (dissected at E6+21h and resulting from tamoxifen administration at E6+5h) and at the OB-EB stage (dissected at E7+3h and resulting from tamoxifen administration at E6+21h). (B, C) Expression heat map of marker genes. Scale indicates z-scored expression values. The data can be found in S7 Source Data. (D) UMAP plot showing the integrated data from the 2 scRNA-seq mid-late streak and OB/EB datasets (see also S11 Fig). Colour codes correspond to the embryonic stage of collection or population identity. The data underlying (D) can be found in S7 Source Data. (E) Expression heat map of marker genes comparing the aPS and primitive streak cells at the mid-late streak stages and primitive streak cells at the OB-EB stages (S7 Source Data). Scale indicates z-scored expression values. (F) UMAP plot of the aPS, MS-LS primitive streak, and OB-EB primitive streak cells colour coded. (G, H) Violin plots showing the normalised log2 expression value of Eomes and T (G) and Foxa2, Lhx1, Sp8 Hes1, Ccnd2, and Notch2 (H) in the aPS and primitive streak at MS-LS and OB-EB stages. The data underlying (G, H) can be found in S7 Source Data. aPS, anterior primitive streak; DE, definitive endoderm; EB, early bud; LPM/Ex-meso, lateral plate mesoderm and extraembryonic mesoderm, mesenchyme; LS, late streak; MS, mid-streak; Nascent meso, nascent mesoderm; OB, no bud; PGC, primordial germs cells; PS, primitive streak; scRNA-seq, single-cell RNA sequencing; UMAP, Uniform Manifold Approximation Projection. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33999917), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Cerberus 1 Antibody (225807)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Cerberus 1 (Catalog # 1986-CB)

Reviewed Applications

Read 1 review rated 5 using MAB1986 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

References

  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  3. Biben, C. et al. (1998) Dev. Biol. 194:135.
  4. Piccolo, S. et al. (1999) Nature 397:707.
  5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
  6. Tavares, A.T. et al. (2007) Development 134:2051.
  7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
  8. Bouwmeester, T. et al. (1996) Nature 382:595.
  9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
  10. Glinka, A. et al. (1997) Nature 389:517.
  11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
  12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
  13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.

Alternate Names

CER1, DAND4

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Gene Symbol

CER1

UniProt

O55233

Additional Cerberus 1 Products

Product Documents for Mouse Cerberus 1 Antibody (225807)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cerberus 1 Antibody (225807)

For research use only

Related Research Areas

BMP Family

Citations for Mouse Cerberus 1 Antibody (225807)

Customer Reviews for Mouse Cerberus 1 Antibody (225807) (1)

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  • Mouse Cerberus 1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E6.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 10/26/2015
    E6.5 mouse embryo fixed in 4% PFA was stained with this antibody. DAPI was used for nuclear counterstain. Cerberus1 positive cells were seen in the anterior visceral endoderm. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS-T<br />Dilution: 1:100
    Mouse Cerberus 1 Antibody (225807) MAB1986

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