Mouse CRELD2 Antibody

Detection of CRELD2 by Western Blot

Lack of CRELD2 effects in murine embryonic fibroblasts.(a) EdU incorporation in mouse embryonic fibroblasts (MEFs) cultured for 24 hours in the absence or presence of transforming growth factor beta 1 (TGF beta 1, 2 ng/mL) or CRELD2 (100 ng/mL). 3–6 experiments. (b) Recovery 16 hours after scratch injury of MEF monolayers in the absence or presence of TGF beta 1 or CRELD2. 4 experiments. (c) Smooth muscle actin alpha 2 (Acta2) and (d) collagen type I alpha1 (Col1a1) mRNA expression (RT-qPCR, normalized to glyceraldehyde-3-phosphate dehydrogenase [Gapdh]) in MEFs cultured for 24 hours in the absence or presence of TGF beta 1 or CRELD2. 6 experiments. (a–d) *P < 0.05, **P < 0.01, ***P < 0.001 (1-way ANOVA with Tukey test). Individual data points, mean values, and SEMs are shown. (e) Exemplary immunoblots (out of 3) showing phosphorylated (P)-SMAD2 (S465/S467), P-SMAD3 (S423/S425), and SMAD2 and SMAD3 expression in MEFs stimulated with TGF beta 1 in the absence or presence of CRELD2 (added 30 min prior to TGF beta 1).Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39196188), licensed under a CC-BY license. Not internally tested by R&D Systems.