Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

R&D Systems | Catalog # MAB4531

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Neutralization, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 124014
View all CXCL1/GRO alpha/KC/CINC-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
Arg20-Lys96
Accession # P12850

Specificity

Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, rrCXCL5, or recombinant porcine CXCL8.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.

Chemotaxis Induced by CXCL1/KC and Neutralization by Mouse CXCL1/KC Antibody.

Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND50 is typically 1-5 µg/mL.

Applications for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha /KC/CINC-1 aa 20-96 (Catalog # 453-KC)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes,  mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

UniProt

P12850

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

For research use only

Citations for Mouse CXCL1/GRO alpha/KC/CINC-1 Antibody (124014)

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