Mouse Cyr61/CCN1 Antibody (466305)

R&D Systems | Catalog # MAB4864

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 466305
View all Cyr61/CCN1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Cyr61/CCN1
Asp176-Gly281
Accession # P18406

Specificity

Detects mouse Cyr61/CCN1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cyr61 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Cyr61/CCN1 Antibody (466305)

Cyr61/CCN1 antibody in Mouse Trigeminal Ganglia by Immunohistochemistry (IHC-Fr).

Cyr61/CCN1 in Mouse Trigeminal Ganglia.

Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Cyr61/CCN1 by Immunocytochemistry/ Immunofluorescence

Detection of Cyr61/CCN1 by Immunocytochemistry/ Immunofluorescence

CYR61 promotes vascular maturation with or without loading in vitro.a Experimental setup highlighting the components of the 3D vascular network-on-a-chip system. The devices were loaded longitudinally (as indicated by the arrows), ensuring that all cells in the vascular compartment (green) experienced uniform, unidirectional compression with consistent strain. Created in BioRender. Boerckel, J. (2025) biorender.com/n15u686. b Representative images. Quantifications of (c) vessel length, (d) vascular area, (e) number of vessel junctions, and (f) number of vessel endpoints as measure for vascular connectivity (n = 3). Absolute values are given for the whole chip area. Mean ± SEM and individual data points. Two-way ANOVA was used to determine the statistical significance; p-values from multiple comparisons are indicated with *p < 0.05; **p< 0.01; ***p < 0.001. Scale bars indicate 500 µm. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41536-025-00398-y), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Cyr61/CCN1 by Immunohistochemistry

Detection of Cyr61/CCN1 by Immunohistochemistry

Spatial expression of YAP, CCN1/CYR61, EMCN, and OSX in the fractured bone at 14 dpf.a Exemplary Movat’s pentachrome staining 14 dpf; black box indicates ROI as magnified in b–d. b Magnification of fracture gap and adjacent bone marrow areas (ROI from (a); black box indicates ROI for (g). c, d Overview stainings. e, f Quantifications (n = 6). One-way repeated measures ANOVA was used to determine the statistical significance; p-values are indicated with *p < 0.05; ***p < 0.001. g Magnifications from endochondral part of fracture gap. Scale bars indicate 1 mm (a) and 500 µm (b–d) and 100 µm (g). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41536-025-00398-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Cyr61/CCN1 Antibody (466305)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse trigeminal ganglia

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4864 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyr61/CCN1

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins  alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

UniProt

P18406

Additional Cyr61/CCN1 Products

Product Documents for Mouse Cyr61/CCN1 Antibody (466305)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cyr61/CCN1 Antibody (466305)

For research use only

Citations for Mouse Cyr61/CCN1 Antibody (466305)

Customer Reviews for Mouse Cyr61/CCN1 Antibody (466305) (2)

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  • Mouse Cyr61/CCN1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: C3H10T1/2 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 05/20/2019
  • Mouse Cyr61/CCN1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 07/15/2018
    Mouse Cyr61/CCN1 Antibody (466305) MAB4864

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