Mouse DEC-205/CD205 Antibody

R&D Systems | Catalog # AF5975

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DEC-205/CD205 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse DEC-205/CD205
Cys216-Pro503
Accession # Q60767

Specificity

Detects mouse DEC-205/CD205 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DEC-205 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse DEC-205/CD205 Antibody

Detection of Mouse DEC‑205/CD205 antibody by Western Blot.

Detection of Mouse DEC‑205/CD205 by Western Blot.

Western blot shows lysates of JAWSII mouse immature dendritic cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC-205/CD205 at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse DEC-205/CD205 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: JAWSII mouse immature dendritic cell line and mouse thymus tissue

Reviewed Applications

Read 1 review rated 5 using AF5975 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DEC-205/CD205

DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.

Alternate Names

CD205, DEC205, Ly75

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Gene Symbol

LY75

UniProt

Q60767

Additional DEC-205/CD205 Products

Product Documents for Mouse DEC-205/CD205 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse DEC-205/CD205 Antibody

For research use only

Customer Reviews for Mouse DEC-205/CD205 Antibody (1)

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Customer Images

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  • Mouse DEC-205/CD205 Antibody
    Name: Jesse Davidson
    Application: Western Blot
    Sample Tested: primary antibody
    Species: Mouse
    Verified Customer | Posted 07/27/2018
    Nitrocellulose membrane probed with 1:200 dilution of Goat Anti-Mouse DEC‑205/CD205 Ab (Catalog # AF5975) followed by HRP-conjugated Anti-Goat IgG Secondary Ab. Reducing conditions; TBST + 5% milk for blocking and incubation steps.
    Antibody used at 1:200 Blocking and incubation in TBST + 5% milk
    Mouse DEC-205/CD205 Antibody AF5975

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail