Mouse EGF Antibody (262930)

R&D Systems | Catalog # MAB2028

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 262930
View all EGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse EGF
Asn977-Arg1029
Accession # P01132

Specificity

Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha, rhNRG1-beta 1, or rhTGF-alpha.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse EGF Antibody (262930)

Mouse EGF ELISA Standard Curve

Mouse EGF ELISA Standard Curve

Recombinant Mouse EGF (Catalog # 2028-EG) was serially diluted and captured by Rat Anti-Mouse EGF Monoclonal Antibody (Catalog # MAB2028) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse EGF Antibody (262930)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse EGF (Catalog # 2028-EG)

Mouse EGF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse EGF Biotinylated Antibody (Catalog # BAF2028)
  • Standard: Recombinant Mouse EGF Protein, CF (Catalog # 2028-EG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

Epidermal Growth Factor (EGF) is the prototypic member of a family of growth factors that have EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

UniProt

P01132

Additional EGF Products

Product Documents for Mouse EGF Antibody (262930)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EGF Antibody (262930)

For research use only

Citations for Mouse EGF Antibody (262930)

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