Mouse EGFR Antibody [Biotin]

R&D Systems | Catalog # BAF1280

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Ferret, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EGFR
Leu25-Ser647
Accession # Q01279

Specificity

Detects mouse EGFR in Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) EGFR is observed and less than 1% cross‑reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EGFR Antibody [Biotin]

EGFR antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

EGFR in Mouse Embryo.

EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1280) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse EGFR by Immunocytochemistry/ Immunofluorescence

Detection of Mouse EGFR by Immunocytochemistry/ Immunofluorescence

BMP7-SMAD signaling inhibits EGFR expression. (A and B) IUE of vectors that express either GFP (control) or Bmp7-GFP in E14.5 WT mouse cortex. At E17.5, EGFR expression was significantly downregulated and pSMAD1/5/9 expression was strongly upregulated in the Bmp7-IUE cortex (arrows). (C) In the hGFAP-Cre;Smad4F/F cortex, Bmp7-IUE showed less EGFR inhibition. (D) Quantification of numbers of EGFR-expressing cells in the cortex. (E) Bmp7 was overexpressed in the E14.5 or E15.5 cortex using IUE. The cortical GLI3R to GLI3FL ratio was significantly increased 48 h later (see Western blot in Fig. S8). Image collected and cropped by CiteAb from the following open publication (https://academic.oup.com/proteincell/article/15/1/21/7193717), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse EGFR by Immunocytochemistry/ Immunofluorescence

Detection of Mouse EGFR by Immunocytochemistry/ Immunofluorescence

BMP7-SMAD signaling inhibits EGFR expression. (A and B) IUE of vectors that express either GFP (control) or Bmp7-GFP in E14.5 WT mouse cortex. At E17.5, EGFR expression was significantly downregulated and pSMAD1/5/9 expression was strongly upregulated in the Bmp7-IUE cortex (arrows). (C) In the hGFAP-Cre;Smad4F/F cortex, Bmp7-IUE showed less EGFR inhibition. (D) Quantification of numbers of EGFR-expressing cells in the cortex. (E) Bmp7 was overexpressed in the E14.5 or E15.5 cortex using IUE. The cortical GLI3R to GLI3FL ratio was significantly increased 48 h later (see Western blot in Fig. S8). Image collected and cropped by CiteAb from the following open publication (https://academic.oup.com/proteincell/article/15/1/21/7193717), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse EGFR Antibody [Biotin]

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)  

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EGFR Fc Chimera (Catalog # 1280-ER)

Reviewed Applications

Read 1 review rated 4 using BAF1280 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as Her1, ErbB1, or ErbB), ErbB2 (Neu, Her2), ErbB3 (Her3), and ErbB4 (Her4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Avivi, A. et al. (1991) Oncogene 6:673.
  4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  5. Reiter J.L. et al. (2001) Genomics 71:1.
  6. Xu, Y.H. et al. (1984) Nature 309:806.
  7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  13. Miettinen, P.J. et al. (1995) Nature 376:337.
  14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

Q01279

Additional EGFR Products

Product Documents for Mouse EGFR Antibody [Biotin]

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Product Specific Notices for Mouse EGFR Antibody [Biotin]

For research use only

Citations for Mouse EGFR Antibody [Biotin]

Customer Reviews for Mouse EGFR Antibody [Biotin] (1)

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  • Mouse EGF R/ErbB1 Biotinylated Antibody
    Name: Rika Ohkubo
    Application: Flow Cytometry
    Sample Tested: Neural progenitor cells
    Species: Mouse
    Verified Customer | Posted 02/23/2016
    I used this antibody with 1:200 dilution (each sample included 10x106 cells).
    Mouse EGFR Antibody [Biotin] BAF1280

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