Mouse EMMPRIN/CD147 Antibody

R&D Systems | Catalog # AF772

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652

Specificity

Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EMMPRIN/CD147 Antibody

Detection of Mouse EMMPRIN/CD147 antibody by Western Blot.

Detection of Mouse EMMPRIN/CD147 by Western Blot.

Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse EMMPRIN/CD147 antibody by Simple WesternTM.

Detection of Mouse EMMPRIN/CD147 by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse EMMPRIN/CD147 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse thymocytes

Simple Western

2 µg/mL
Sample: Mouse brain (cortex) tissue and mouse heart tissue

Western Blot

0.2 µg/mL
Sample: Mouse brain tissue, mouse heart tissue, and mouse small intestine tissue

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMMPRIN/CD147

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Entrez Gene IDs

682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)

Gene Symbol

BSG

UniProt

NP_001070652

Additional EMMPRIN/CD147 Products

Product Documents for Mouse EMMPRIN/CD147 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EMMPRIN/CD147 Antibody

For research use only

Citations for Mouse EMMPRIN/CD147 Antibody

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