Mouse Endosialin/CD248 Antibody (458606)

R&D Systems | Catalog # MAB7535

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 458606
View all Endosialin/CD248 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endosialin/CD248
Val13-Arg693 (predicted)
Accession # Q91V98

Specificity

Detects mouse Endosialin/CD248 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endosialin/CD248 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Endosialin/CD248 Antibody (458606)

Endosialin/CD248 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC).

Endosialin/CD248 in bEnd.3 Mouse Cell Line.

Endosialin/CD248 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Endosialin/CD248 antibody in IMR-32 Human Cell Line by Immunocytochemistry (ICC).

Endosialin/CD248 in IMR-32 Human Cell Line.

Endosialin/CD248 was detected in immersion fixed IMR-32 human neuroblastoma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Endosialin/CD248 Antibody (458606)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line and IMR-32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endosialin/CD248

Endosialin, also known as CD248 and tumor endothelial marker 1 (Tem1), is a 165 kDa transmembrane O-glycosylated protein that contains one C-type lectin, one Sushi, one EGF-like domain and a mucin-like stalk in its extracellular domain (ECD). It is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature but is downregulated in quiescent vasculature. Endosialin regulates pericyte proliferation, migration, and adhesion to matrix Fibronectin and Collagens I and IV. It is also required for lymph node and spleen remodeling during immune responses. Within the ECD, mouse Endosialin shares 76% and 93% aa sequence identity with human and rat Endosialin, respectively.

Alternate Names

CD164L1, CD248, TEM1

Entrez Gene IDs

57124 (Human); 70445 (Mouse); 293669 (Rat)

Gene Symbol

CD248

UniProt

Q91V98 (Mouse)

Additional Endosialin/CD248 Products

Product Documents for Mouse Endosialin/CD248 Antibody (458606)

Certificate of Analysis

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Product Specific Notices for Mouse Endosialin/CD248 Antibody (458606)

For research use only

Citations for Mouse Endosialin/CD248 Antibody (458606)

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