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Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 666521
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
Thr23-Thr653
Accession # P70424
Thr23-Thr653
Accession # P70424
Specificity
Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with
recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse ErbB2/Her2 Antibody (666521)
Detection of ErbB2 in 3T3-LI Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 Monoclonal Antibody (Catalog # MAB6744, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).Applications for Mouse ErbB2/Her2 Antibody (666521)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Reviewed Applications
Read 1 review rated 5 using MAB6744 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
UniProt
Additional ErbB2/Her2 Products
Product Documents for Mouse ErbB2/Her2 Antibody (666521)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse ErbB2/Her2 Antibody (666521)
For research use only
Related Research Areas
Customer Reviews for Mouse ErbB2/Her2 Antibody (666521) (1)
5 out of 5
1 Customer Rating
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Application: Flow CytometrySample Tested: 4T1 mouse breast cancer cell lineSpecies: MouseVerified Customer | Posted 11/01/2021Detection of mouse HER2 on the 4T1 breast cancer mouse cell line. 4T1 cells were treated with the Mouse ErbB2/Her2 antibody (catalog # MAB6744) (BLUE) or Rat IgG1 isotype control antibody (RED), followed by a secondary Goat Anti-Rat IgG APC antibody.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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Associated Pathways
