Mouse ESGP Antibody

R&D Systems | Catalog # AF4580

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Golden Syrian Hamster, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ESGP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ESGP
Leu24-Lys84
Accession # Q2Q5T5

Specificity

Detects mouse ESGP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse ESGP Antibody

Detection of Mouse ESGP antibody by Western Blot.

Detection of Mouse ESGP by Western Blot.

Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ESGP at approximately 9 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
ESGP antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

ESGP in Mouse Testis.

ESGP was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to late spermatids. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
ESGP antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

ESGP in D3 Mouse Cell Line.

ESGP was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse ESGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4580) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of ESGP by Western Blot

Detection of ESGP by Western Blot

Validation of transgenic models to activate Myomaker and Myomerger expression in myofibers. A Schematic of the breeding strategy for inducible expression of Myomaker within myofibers. B Schematic of the breeding strategy for inducible expression of Myomerger within myofibers. C qPCR analysis of Myomaker mRNA levels from the TA, rectus femoris, and gastroc muscles after 3 days of dox treatment. D qPCR analysis for Myomerger mRNA levels from the TA, rectus femoris, and gastroc muscles after 3 days of induction. E Western blot for Myomaker expression in the soleus, EDL, TA, gastroc, and rectus femoris after 3 days of dox treatment. F Western blot for Myomerger expression in the soleus, EDL, TA, gastroc, and rectus femoris after 3 days of dox treatment. G Western blot for Myomaker expression in the gastroc muscle after 2 weeks of induction. H Western blot for Myomerger in the gastroc after 2 weeks of induction. Statistical analyses and presentation: data are presented as mean ± SEM. C and D Two-tailed Student’s t-test; *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37127758), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ESGP by Western Blot

Detection of ESGP by Western Blot

Validation of transgenic models to activate Myomaker and Myomerger expression in myofibers. A Schematic of the breeding strategy for inducible expression of Myomaker within myofibers. B Schematic of the breeding strategy for inducible expression of Myomerger within myofibers. C qPCR analysis of Myomaker mRNA levels from the TA, rectus femoris, and gastroc muscles after 3 days of dox treatment. D qPCR analysis for Myomerger mRNA levels from the TA, rectus femoris, and gastroc muscles after 3 days of induction. E Western blot for Myomaker expression in the soleus, EDL, TA, gastroc, and rectus femoris after 3 days of dox treatment. F Western blot for Myomerger expression in the soleus, EDL, TA, gastroc, and rectus femoris after 3 days of dox treatment. G Western blot for Myomaker expression in the gastroc muscle after 2 weeks of induction. H Western blot for Myomerger in the gastroc after 2 weeks of induction. Statistical analyses and presentation: data are presented as mean ± SEM. C and D Two-tailed Student’s t-test; *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37127758), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ESGP by Western Blot

Detection of ESGP by Western Blot

Inhibition of MuSC late differentiation by administration of recombinant CCN2/CTGF protein or CCN2 overexpression. (A–D) MuSCs were isolated from adult mice and treated with different concentrations of recombinant CTGF protein or control in vitro. (A) BrdU incorporation in the control and CTGF protein supplement groups. Recombinant CTGF protein was administered to MuSCs isolated from adult mice for 2 days, with BrdU added 24 h before sample harvest. (B) Immunofluorescence staining of MyoG representing autonomously differentiated MuSCs in the control and CTGF‐treated groups. MuSCs were cultured with growth medium with/without CTGF supplement and quantification results. Scale bar, 100 μm. (C) IF staining of MyoG after 4 days of differentiation and quantification results, including the fusion index after 4 days of differentiation (n = 5 per group in panels B and C). Scale bar, 50 μm. (D) MyoG and Myomixer western blot results. MyoG and Myomixer protein expression levels were normalized to tubulin (n = 3 per group). (E, F) MuSCs from adult mice were transfected with a control (Lenti‐CON) or CCN2‐overexpressed (Lenti‐CCN2) lentivirus. (E) Four days after differentiation, cells were stained with MHC and DAPI, and the fusion index was quantified as myotubes with three or more nuclei/total nuclei number (n = 6 per group). Scale bar, 50 μm. (F) Western blot results of MHC normalized to GAPDH (n = 3 per group). Data are presented as mean ± SD; one‐way ANOVA was used in panels (A) and (B); and a t‐test was used in panels (C)–(F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38925632), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse ESGP Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testes

Western Blot

0.1 µg/mL
Sample: D3 mouse embryonic stem cell line

Reviewed Applications

Read 1 review rated 5 using AF4580 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ESGP

ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family. It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation. Mouse ESGP precursor is 84 amino acids (aa) in length. It contains a 25 aa signal sequence with a 59 aa mature segment. There are no N-linked glycosylation sites. One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site.

Long Name

Embryonic Stem Cell and Germ Cell Specific Protein

Alternate Names

EG653016

Entrez Gene IDs

653016 (Mouse)

Gene Symbol

MYMX

UniProt

Q2Q5T5

Additional ESGP Products

Product Documents for Mouse ESGP Antibody

Certificate of Analysis

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Product Specific Notices for Mouse ESGP Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Mouse ESGP Antibody

Customer Reviews for Mouse ESGP Antibody (1)

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  • Mouse ESGP Antibody
    Name: Anonymous
    Application: Knockout Validated
    Sample Tested: Muscle tissue
    Species: Mouse
    Verified Customer | Posted 10/16/2020
    Western blotting analysis of Myomixer expression in gastrocnemius muscles at day 3 post-injury. Cat# AF4580, 1:1,000 dilution.
    Specificity validated by KO samples
    Mouse ESGP Antibody AF4580
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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