Mouse ETV2/ER71 Antibody (827940)

R&D Systems | Catalog # MAB7740

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 827940
View all ETV2/ER71 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ETV2/ER71
Asn125-Arg231
Accession # P41163

Specificity

Detects mouse ETV2/ER71 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse ETV2/ER71 Antibody (827940)

Detection of Mouse ETV2/ER71 antibody by Western Blot.

Detection of Mouse ETV2/ER71 by Western Blot.

Western blot shows lysates of bEnd.3 mouse endothelioma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse ETV2/ER71 Monoclonal Antibody (Catalog # MAB7740) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ETV2/ER71 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse ETV2/ER71 Antibody (827940)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: bEnd.3 mouse endothelioma cell line and D3 mouse embryonic stem cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ETV2/ER71

ETS translocation variant 2 (ETV2), also known as ETSRP and ER71, is a 37 kDa transcription factor that is expressed in presumptive vascular progenitor cells. It plays an important role in vascular development by promoting the expansion of hemangioblasts and vascular endothelial cells while inhibiting differentiation into cardiomyocytes. It contains one ETS DNA binding domain (aa 234-314). Within aa 125-231, mouse ETV2 shares 54% and 85% aa sequence identity with human and rat ETV2, respectively.

Long Name

ETS Translocation 2

Alternate Names

ER71, ETSRP71

Entrez Gene IDs

2116 (Human); 14008 (Mouse); 361544 (Rat)

Gene Symbol

ETV2

UniProt

P41163 (Mouse)

Additional ETV2/ER71 Products

Product Documents for Mouse ETV2/ER71 Antibody (827940)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ETV2/ER71 Antibody (827940)

For research use only

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