Mouse FAM3D Antibody

Detection of FAM3D by Western Blot

Expression of Fam3d in the uterus of ovariectomized (OVX) mice following treatment with E2. (A) RT-PCR and qRT-PCR analysis of Fam3d in OVX mouse uterus at 2, 4, 6, 12, and 24 h post-E2 treatment. Ribosomal protein L7 (Rpl7) was used as an internal control. Standard error of the mean (SEM) was calculated for each group (n = 3). The fold changes were determined by comparing the mRNA expression levels at each time point to the levels of 0 h samples. The statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (*** p < 0.001 versus 0 h). (B) Western blot analysis of FAM3D in OVX mouse uterus at 2, 4, 6, 12, and 24 h post-E2 treatment. beta -actin was used as an internal control. Relative intensities were normalized to that of beta -actin. SEM was calculated for each group (n = 3). The fold changes were evaluated by comparing the expression levels at each time point to the levels of 0 h samples. The statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (*** p < 0.001 versus 0 h). (C) Immunofluorescence staining of the OVX mice uteri at 2, 4, 6, 12, and 24 h after E2 treatment using FAM3D antibody (n = 3). Red represents FAM3D and blue represents DAPI. Scale bar, 20 μm. LE, luminal epithelium; GE, glandular epithelium; S, stroma. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/26/24/11840), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FAM3D by Western Blot

Expression of Fam3 family in different mouse organs and the uterus during estrous cycle. (A) RT-PCR analysis of Fam3 family in different mouse tissues. Ribosomal protein L7 (Rpl7) was used as an internal control. (B) RT-PCR analysis of Fam3 family in mouse uterus during each stage of the estrous cycle. Rpl7 was used as an internal control. (C) qRT-PCR analysis of Fam3 family in mice uteri for each stage of the estrous cycle. Standard error of the mean (SEM) was calculated for each group (n = 3). The fold changes were evaluated by comparing the mRNA expression level at each stage to Fam3d expression at diestrus. The statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (* p < 0.05). (D) Western blot analysis of FAM3D in mouse uterus during each stage of the estrous cycle. beta -actin was used as an internal control. (E) Relative intensity of FAM3D in mouse uterus during each stage of the estrous cycle. Relative intensities were normalized to that of beta -actin. SEM was calculated for each group (n = 3). The fold changes were evaluated by comparing the expression level at each stage to that at diestrus. The statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test (* p < 0.05). (F) Immunofluorescence staining of mouse uterus at each stage during the estrous cycle using FAM3D antibody (n = 3). Red represents FAM3D and blue represents DAPI. Scale bar, 20 μm. LE, luminal epithelium; GE, glandular epithelium; S, stroma; P, proestrus; E, estrus; M, metestrus; D, diestrus. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/26/24/11840), licensed under a CC-BY license. Not internally tested by R&D Systems.