Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

R&D Systems | Catalog # MAB9727

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 983802
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fibroblast Activation Protein  alpha /FAP
Leu26-Asp761
Accession # P97321

Specificity

Detects mouse Fibroblast Activation Protein  alpha /FAP in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

Detection of Fibroblast Activation Protein alpha/FAP antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Fibroblast Activation Protein alpha/FAP Monoclonal Antibody (Catalog # MAB9727, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Fibroblast Activation Protein a/FAP antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Fibroblast Activation Protein alpha /FAP in C2C12 Mouse Cell Line.

Fibroblast Activation Protein a/FAP was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB9727) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Mouse Fibroblast Activation Protein  alpha /FAP Antibody Detection of Fibroblast Activation Protein alpha/FAP in NIH-3T3 Mouse Embryonic Fibroblast Cell Line by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha/FAP in NIH-3T3 Mouse Embryonic Fibroblast Cell Line by Flow Cytometry.

Used 3uL for 15mins at 4°C on 3T3 cells transduced to over express murine FAP. Image from a verified customer review.
Detection of Fibroblast Activation Protein alpha /FAP by Western Blot

Detection of Fibroblast Activation Protein alpha /FAP by Western Blot

FAP expression is up-regulated in the myocardium of isoproterenol (ISO) mice and in cardiac fibroblasts upon TGF-beta 1 stimulation. (A) Quantitative polymerase chain reaction (qPCR) analysis showing messenger RNA (mRNA) expression levels of Col1 alpha 1, Acta2, and FAP in heart tissue from ISO-treated mice. n = 6. Data are presented as mean ± SEM. **P < 0.01; ****P < 0.0001. (B) Western blotting analysis of Col1 alpha 1, alpha -smooth muscle actin ( alpha -SMA), and FAP protein expressions in the hearts of mice treated with ISO or saline. (C) Sirius Red staining and immunohistochemical staining for FAP in hearts from ISO-treated mice. (D) mRNA expression levels of Col1 alpha 1, Acta2, and FAP in neonatal rat cardiac fibroblasts (NRCFs) treated with TGF-beta 1 (10 ng/ml) for 24 h, assessed by qPCR. (E) Western blotting analysis of FAP protein expression in NRCFs after TGF-beta 1 treatment. (F) Flow cytometry analysis of FAP protein expression in NRCFs following TGF-beta 1 treatment. (G) Immunofluorescence (IF) analysis of FAP protein expression in NRCFs post-TGF-beta 1 treatment. IHC, immunohistochemical; Col I, type I collagen; PBS, phosphate-buffered saline; Ctrl, control; GAPDH, glyceraldehyde-3-phosphate; DAPI, 4′,6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40225952), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibroblast Activation Protein alpha /FAP by Western Blot

Detection of Fibroblast Activation Protein alpha /FAP by Western Blot

FAP expression is up-regulated in the myocardium of isoproterenol (ISO) mice and in cardiac fibroblasts upon TGF-beta 1 stimulation. (A) Quantitative polymerase chain reaction (qPCR) analysis showing messenger RNA (mRNA) expression levels of Col1 alpha 1, Acta2, and FAP in heart tissue from ISO-treated mice. n = 6. Data are presented as mean ± SEM. **P < 0.01; ****P < 0.0001. (B) Western blotting analysis of Col1 alpha 1, alpha -smooth muscle actin ( alpha -SMA), and FAP protein expressions in the hearts of mice treated with ISO or saline. (C) Sirius Red staining and immunohistochemical staining for FAP in hearts from ISO-treated mice. (D) mRNA expression levels of Col1 alpha 1, Acta2, and FAP in neonatal rat cardiac fibroblasts (NRCFs) treated with TGF-beta 1 (10 ng/ml) for 24 h, assessed by qPCR. (E) Western blotting analysis of FAP protein expression in NRCFs after TGF-beta 1 treatment. (F) Flow cytometry analysis of FAP protein expression in NRCFs following TGF-beta 1 treatment. (G) Immunofluorescence (IF) analysis of FAP protein expression in NRCFs post-TGF-beta 1 treatment. IHC, immunohistochemical; Col I, type I collagen; PBS, phosphate-buffered saline; Ctrl, control; GAPDH, glyceraldehyde-3-phosphate; DAPI, 4′,6-diamidino-2-phenylindole. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40225952), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Fibroblast Activation Protein  alpha /FAP Monoclonal Antibody (Catalog # MAB97271).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB9727 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).

References

  1. Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
  2. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  3. Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
  4. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  5. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
  7. Bauer, S. et al. (2006) Arthritis Res. 8:R171.
  8. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
  9. Keane, F.M. et al. (2011) FEBS J. 278:1316.
  10. Ghersi, G. et al. (2006) Cancer Res. 66:4652.
  11. Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
  12. Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
  13. Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
  14. Kraman, M. et al. (2010) Science 330:827.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Gene Symbol

FAP

UniProt

P97321

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

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Product Specific Notices for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

For research use only

Citations for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802)

Customer Reviews for Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802) (1)

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  • Mouse Fibroblast Activation Protein  alpha /FAP Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 08/26/2025
    Used 3uL for 15mins at 4°C on 3T3 cells transduced to over express murine FAP. Other murine FAP antibodies give minimal shift but this AF647-conjugated antibodies gave a really terrific shift
    Mouse Fibroblast Activation Protein alpha/FAP Antibody (983802) MAB9727

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