Mouse HSP10/EPF Antibody [Biotin]

R&D Systems | Catalog # BAF2584

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all HSP10/EPF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HSP10/EPF
Ala2-Asp102
Accession # Q64433

Specificity

Detects endogenous levels of mouse HSP10/EPF in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse HSP10/EPF Antibody [Biotin]

Detection of Mouse HSP10/EPF antibody by Western Blot.

Detection of Mouse HSP10/EPF by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse HSP10/EPF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse HSP10/EPF Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and DA3 mouse myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10.

Long Name

Heat Shock 10 kDa Protein 1

Alternate Names

Chaperonin 10 Homolog, EPF, GroES, HSPE1

Entrez Gene IDs

3336 (Human); 69253 (Mouse)

Gene Symbol

HSPE1

UniProt

Q64433

Additional HSP10/EPF Products

Product Documents for Mouse HSP10/EPF Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse HSP10/EPF Antibody [Biotin]

For research use only

Related Research Areas

Heat Shock Proteins (HSPs)

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