Mouse IGF-I/IGF-1 Antibody [Biotin]

R&D Systems | Catalog # BAF791

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IGF-I/IGF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IGF-I/IGF-1
Gly33-Ala102
Accession # Q8CAR0

Specificity

Detects mouse IGF-I/IGF-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IGF-I/IGF-1 is observed and less than 0.1% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IGF-I/IGF-1 Antibody [Biotin]

Mouse / Rat IGF-I ELISA Standard Curve

Mouse / Rat IGF-I ELISA Standard Curve

Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse IGF-I/IGF-1 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)

Mouse IGF-I/IGF-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse IGF-I/IGF-1 Antibody (Catalog # MAB791)
  • Standard: Recombinant Mouse IGF-I/IGF-1 Protein, CF (Catalog # 791-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF791 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

Q8CAR0

Additional IGF-I/IGF-1 Products

Product Documents for Mouse IGF-I/IGF-1 Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Mouse IGF-I/IGF-1 Antibody [Biotin]

For research use only

Citations for Mouse IGF-I/IGF-1 Antibody [Biotin]

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  • Mouse IGF‑I Biotinylated Antibody
    Name: Alexandria Schieber
    Application: ELISA
    Sample Tested: Serum, Adipose tissue, Adipocytes, Liver tissue, Large intestine and Small intestine tissue
    Species: Mouse and Bacterial
    Verified Customer | Posted 02/22/2016
    Homemade ELISA, used R and D systems capture antibody and purified protein

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