Mouse IL-13 Antibody (38213)

R&D Systems | Catalog # MAB413

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, Cell Culture, ELISA Development, ELISA Development (Capture), In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 38213
View all IL-13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-13
Accession # P20109

Specificity

Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IL-13 Antibody (38213)

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.

Recombinant Mouse IL-13 (Catalog # 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-13 Monoclonal Antibody (Catalog # MAB413). The ND50 is typically 0.3-0.8 µg/mL.
Mouse IL-13 ELISA Standard Curve

Mouse IL-13 ELISA Standard Curve

Recombinant Mouse IL‑13 (Catalog # 413-ML) was serially diluted and captured by Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of IL-13 by Immunohistochemistry

Detection of IL-13 by Immunohistochemistry

Pharmacologic inhibition of the tuft cell-ILC2 circuit reduces gastric tumor growth.a Schematic outline of the antibody-mediated blockade of IL13 or IL25. 13-week-old mice were treated with either alpha -IL13, alpha -IL25 or a matched IgG isotype control (300 μg/20 g mouse, once weekly for 3 weeks). Mice were culled one week after the third injection. EP = endpoint. b Representative images of gp130F/F stomachs treated as described in Fig. 3a. Dotted circles indicate tumors. Scale bar = 8 mm. c Tumor mass of gp130F/F mice following treatment with alpha -IL13 or a matched IgG isotype control. N = 6 and 6 respectively. d Tumor mass of gp130F/F mice following treatment with alpha -IL25 or a matched IgG isotype control. N = 6 and 6 respectively. e IHC staining and quantification of DCLK1+ tuft cells in alpha -IL13 and IgG treated gp130F/F mice. Scale bar = 300 μm. N = 6 and 6 respectively. f IHC staining quantification of DCLK1+ tuft cells in alpha -IL25 and IgG treated gp130F/F mice. Scale bar = 300 μm. N = 6 and 7 respectively. g Flow-cytometry quantification of KLRG1+CD90.2+Lineage− CD45+ ILC2s in tumors of gp130F/F mice treated with alpha -IL13 or a matched IgG isotype control. N = 6 and 6 respectively. h Immunofluorescence (IF) staining and quantification of Gata3+CD3- ILC2s in tumors of gp130F/F mice treated with alpha -IL25 or a matched IgG control. Arrows indicate ILC2s. Scale bar = 100 μm. N = 5 and 5 respectively. Data represents mean ± SEM, p values from two-sided Student’s t-test *p < 0.05, **p < 0.01, ***p < 0.001. Each symbol represents an individual mouse. Data is from two pooled experiments. Source data and exact p values are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37898600), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL-13 Antibody (38213)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑13 (Catalog # 413-ML)

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.3-0.8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.

Mouse IL-13 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑13 Biotinylated Antibody (Catalog # BAF413)
  • Standard: Recombinant Mouse IL-13 Protein (Catalog # 413-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB413 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

UniProt

P20109

Additional IL-13 Products

Product Documents for Mouse IL-13 Antibody (38213)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-13 Antibody (38213)

For research use only

Citations for Mouse IL-13 Antibody (38213)

Customer Reviews for Mouse IL-13 Antibody (38213) (2)

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  • Mouse IL-13 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: TF-1 human erythroleukemic cell line
    Species: Human
    Verified Customer | Posted 09/05/2021
    Mouse IL-13 Antibody (38213) MAB413
  • Mouse IL-13 Antibody
    Name: Leslie Priddy
    Application: ELISA
    Sample Tested: Tumor cell lyastes
    Species: Mouse
    Verified Customer | Posted 04/01/2018

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