Mouse IL-22BP Antibody

R&D Systems | Catalog # AF2376

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IL-22BP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP
Thr19-Pro230 (Asp149Glu)
Accession # Q80XF5

Specificity

Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and approximately 5% cross-reactivity with rhIL-22 R is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse IL-22BP Antibody

Detection of IL-22BP by Western Blot

Detection of IL-22BP by Western Blot

TAM-treated K8flox/flox; CDX2P-CreERT2; Apcflox/+ mice show downregulation of colonic keratins and minor changes in EMT-associated signaling in the colon. (A) Total proximal colon lysates from K8flox/flox; CDX2P-CreERT2; Apcflox/+ –TAM (Lanes 1–3), K8flox/flox; CDX2P-CreERT2; Apcflox/+ +TAM (Lanes 4–6) and (B) total distal colon lysates from K8flox/flox; CDX2P-CreERT2; Apcflox/+ –TAM (Lanes 1–3), K8flox/flox; CDX2P-CreERT2; Apcflox/+ +TAM (Lanes 4–6) on day 78 were immunoblotted for K8, K7, K18, K19, and K20. beta -actin was used as the loading control. (C) Western blots from (A and B) were quantified and normalized to beta -actin. The results are presented as mean (n = 3 mice per group, each data point represents an individual mouse) protein fold changes ± SD. (D) Total proximal colon lysates from K8flox/flox; CDX2P-CreERT2; Apcflox/+ –TAM (Lanes 1–3), K8flox/flox; CDX2P-CreERT2; Apcflox/+ +TAM (Lanes 4–6) and total distal colon lysates from K8flox/flox; CDX2P-CreERT2; Apcflox/+ –TAM (Lanes 7–9), K8flox/flox; CDX2P-CreERT2; Apcflox/+ +TAM (Lanes 10–12) on day 78 were immunoblotted for IL-22BP, p-STAT3, STAT3, p-Smad2, Smad2, Smad4, and Twist. beta -tubulin was used as the loading control. (E) Western blots from (D) were quantified and normalized to beta -tubulin. The results are presented as mean (n = 3 mice per group, each data point represents an individual mouse) protein fold changes ± SD. The statistical significance was determined after performing unpaired Student’s t-test for (C and E), shown as ∗P <.05; ∗∗P <.01; ∗∗∗P <.001; and ∗∗∗∗P <.0001. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2352345X25002589), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL-22BP Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑22BP Fc Chimera (Catalog # 2376-BP)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL‑10, IL-26, IL-28, and IL-29 (1‑4). IL‑10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6‑8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8‑10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL‑22‑induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).

References

  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  4. Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223.
  5. Wolk, K. et al. (2005) Genes Immun. 6:8.
  6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.
  7. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
  8. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  11. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
  12. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
  13. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
 

Long Name

Interleukin 22 Binding Protein

Alternate Names

CRF2-10, CRF2-X, IL-22 RA2, IL22BP, IL22RA2

Entrez Gene IDs

116379 (Human); 237310 (Mouse)

Gene Symbol

IL22RA2

UniProt

Q80XF5 (Sheep)

Additional IL-22BP Products

Product Documents for Mouse IL-22BP Antibody

Certificate of Analysis

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Product Specific Notices for Mouse IL-22BP Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Mouse IL-22BP Antibody

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