Mouse IL-38/IL-1F10 Antibody (798038R)

R&D Systems | Catalog # MAB77742

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 798038R
View all IL-38/IL-1F10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-38/IL-1F10
Met1-Arg152
Accession # Q8R459

Specificity

Detects mouse IL-38/IL-1F10 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL-38/IL-1F10 Antibody (798038R)

Mouse IL-38/IL-1F10 Antibody in ELISA Standard Curve.

Mouse IL‑38/IL‑1F10 ELISA Standard Curve.

Recombinant Mouse IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Mouse IL-38/IL-1F10 Antibody (798038R)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog # MAB7774R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-38/IL1F10 DuoSet ELISA Kit (Catalog # DY2427-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-38/IL-1F10

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Long Name

Interleukin 1 Family Member 10

Alternate Names

FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38

Entrez Gene IDs

84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)

Gene Symbol

IL1F10

UniProt

Q8R459

Additional IL-38/IL-1F10 Products

Product Documents for Mouse IL-38/IL-1F10 Antibody (798038R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-38/IL-1F10 Antibody (798038R)

For research use only

Related Research Areas

IL-1 Family

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FAQs

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Associated Pathways

IL-1 Family Signaling Pathways
IL-1 Family Signaling Pathway Thumbnail