Mouse Integrin alpha 2b/CD41 Antibody (386629)

R&D Systems | Catalog # MAB41181

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 386629
View all Integrin alpha 2b/CD41 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b/CD41
Leu32-Arg988
Accession # NP_034705

Specificity

Detects mouse Integrin alpha 2b/CD41 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Integrin alpha 2b/CD41 Antibody (386629)

Integrin a2b/CD41 antibody in Mouse Bone Marrow Cells by Immunocytochemistry (ICC).

Integrin alpha 2b/CD41 in Mouse Bone Marrow Cells.

Integrin a2b/CD41 was detected in immersion fixed mouse bone marrow cells using Rat Anti-Mouse Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB41181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL014) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Integrin alpha 2b/CD41 in Mouse Platelets by Flow Cytometry.

Mouse platelets were stained with either Rat Anti-Mouse Integrin a2b/CD41 Monoclonal Antibody (MAB41181, closed histogram) or Rat IgG2A Isotype Control (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha 2b/CD41 Antibody (386629)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse platelets

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse platelets and immersion fixed mouse bone marrow cells

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 2b/CD41

Integrin alpha 2b, (ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. ITGA2b associates with integrin beta 3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. ITGA2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively.

Alternate Names

CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)

Gene Symbol

ITGA2B

UniProt

NP_034705

Additional Integrin alpha 2b/CD41 Products

Product Documents for Mouse Integrin alpha 2b/CD41 Antibody (386629)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin alpha 2b/CD41 Antibody (386629)

For research use only

Citations for Mouse Integrin alpha 2b/CD41 Antibody (386629)

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Protocols

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