Mouse Integrin beta 4/CD104 Antibody (308601)

R&D Systems | Catalog # MAB4054

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 308601
View all Integrin beta 4/CD104 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
Asn29-Ser711
Accession # NP_001005608

Specificity

Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Integrin beta 4/CD104 Antibody (308601)

Detection of Mouse Integrin beta 4/CD104 antibody by Western Blot.

Detection of Mouse Integrin beta 4/CD104 by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin beta 4/CD104 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Integrin beta 4/CD104 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry.

Detection of Integrin beta 4/CD104 in CMT-93 Mouse Cell Line by Flow Cytometry.

CMT-93 mouse rectal carcinoma cell line was stained with Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Applications for Mouse Integrin beta 4/CD104 Antibody (308601)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestinal villi

Western Blot

2 µg/mL
Sample: Mouse small intestine tissue

Reviewed Applications

Read 4 reviews rated 4.5 using MAB4054 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 4/CD104

Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin  alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.

Alternate Names

CD104, ITGB4

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Gene Symbol

ITGB4

UniProt

NP_001005608

Additional Integrin beta 4/CD104 Products

Product Documents for Mouse Integrin beta 4/CD104 Antibody (308601)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin beta 4/CD104 Antibody (308601)

For research use only

Citations for Mouse Integrin beta 4/CD104 Antibody (308601)

Customer Reviews for Mouse Integrin beta 4/CD104 Antibody (308601) (4)

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Mouse Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Small intestine tissue
    Species: Mouse
    Verified Customer | Posted 08/20/2022
    Mouse Integrin beta 4/CD104 Antibody (308601) MAB4054
  • Mouse Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate tissue
    Species: Mouse
    Verified Customer | Posted 11/30/2021
    Mouse Integrin beta 4/CD104 Antibody (308601) MAB4054
  • Mouse Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Mouse Integrin beta 4/CD104 Antibody (308601) MAB4054
  • Mouse Integrin  beta 4/CD104 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

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Protocols

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FAQs

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