Mouse Lipocalin-2/NGAL Antibody (228418)

Detection of Mouse Lipocalin‑2/NGAL by Western Blot.

Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Lipocalin-2/NGAL by Immunocytochemistry/ Immunofluorescence

Response of Low-FBS/+FGF2 Cultured Astrocytes to LPS+IFN-gamma Stimulation at the Protein Level(A) Representative western blot illustrating temporal induction of pSTAT3 upon 1 and 48 hr of treatment with LPS+IFN-gamma. (B and C) Representative western blots of activation markers in resting versus LPS+IFN-gamma -stimulated low-FBS astrocytes: (B) Lcn2 expression in resting and 24-hr-stimulated astrocytes; and (C) GFAP and Vim levels under resting conditions and following 48 hr of activation followed by 3 or 5 days of rest. beta -Actin was used as a loading control. (D) Representative immunofluorescence micrographs of GFAP, Vim, and Lcn2 immunoreactivity in resting and activated conditions (GFAP/Vim: green; Lcn2: red; DAPI nuclear stain: blue). Scale bars, 100 μm. (E) Secreted IL-6 as measured by ELISA, resting versus activated conditions, at 48 and 120 hr post-stimulation. Data are expressed as the mean of n ≥ 3 biological replicates ± SD. *p ≤ 0.05, relative to non-activated control samples (multiple t tests with statistical significance determined using the Holm-Sidak method, alpha  = 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29499926), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Lipocalin-2/NGAL by Western Blot

Response of Low-FBS/+FGF2 Cultured Astrocytes to LPS+IFN-gamma Stimulation at the Protein Level(A) Representative western blot illustrating temporal induction of pSTAT3 upon 1 and 48 hr of treatment with LPS+IFN-gamma. (B and C) Representative western blots of activation markers in resting versus LPS+IFN-gamma -stimulated low-FBS astrocytes: (B) Lcn2 expression in resting and 24-hr-stimulated astrocytes; and (C) GFAP and Vim levels under resting conditions and following 48 hr of activation followed by 3 or 5 days of rest. beta -Actin was used as a loading control. (D) Representative immunofluorescence micrographs of GFAP, Vim, and Lcn2 immunoreactivity in resting and activated conditions (GFAP/Vim: green; Lcn2: red; DAPI nuclear stain: blue). Scale bars, 100 μm. (E) Secreted IL-6 as measured by ELISA, resting versus activated conditions, at 48 and 120 hr post-stimulation. Data are expressed as the mean of n ≥ 3 biological replicates ± SD. *p ≤ 0.05, relative to non-activated control samples (multiple t tests with statistical significance determined using the Holm-Sidak method, alpha  = 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29499926), licensed under a CC-BY license. Not internally tested by R&D Systems.