Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

R&D Systems | Catalog # MAB1037

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Bovine, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, COMET, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunodepletion

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # RB6-8C5
View all Ly-6G/Ly-6C (Gr-1) Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Specificity

Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

Detection of Ly-6G/Ly-6C in Frozen Mouse Spleen viaseqIF™ staining on COMET™

Ly-6G/Ly-6C was detected in frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C Monoclonal Antibody (Catalog # MAB1037) at 7ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent preprocessing by incubating tissue with Multi Staining Buffer (Lunaphore Catalog # BU06) for 5 minutes at room temperature followed by a 20 minute incubation in Tris-Buffered Saline + 0.2% Triton at room temperature. Tissue was stained using the Alexa Fluor™ 555 Goat anti-Rat IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
Ly-6G/Ly-6C (Gr-1) antibody in Mouse Spleno-cytes by Immunocytochemistry (ICC).

Ly-6G/Ly-6C (Gr-1) in Mouse Spleno-cytes.

Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Anti-body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Ly-6G/Ly-6C (Gr-1) antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.

Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Ly-6G/Ly-6C (Gr-1) in Mouse Bone Marrow by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R FITC Monoclonal Antibody (FAB1217F) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Monoclonal Antibody (MAB1037) or (B) Rat IgG2B Isotype Control (MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse Mouse Ly-6G/Ly-6C (Gr-1) Antibody by Immunohistochemistry

Detection of Mouse Mouse Ly-6G/Ly-6C (Gr-1) Antibody by Immunohistochemistry

Lymphocyte/neutrophil ratio increases upon NHE1 inhibition in tumor sections of KPfC mice.(A) H&E (left) and PAS-stained KPfC mouse tissue sections after vehicle and gemcitabine + cariporide (GEM+CARI) therapy. Cells of innate immunity, such as neutrophils (arrows), utilize glycogen and are thus PAS+ (purple), in contrast to, for example, lymphocytes. Scale bar: 50 μm. (B) Representative IHC images stained for Ly6G+ neutrophils (magenta, arrows on left image), CD3+ lymphocytes (yellow, arrows on the right image), and nuclei with DAPI (cyan). Scale bar: 50 μm. (C) CD3/Ly6G ratio was assessed by dividing the number of CD3+ cells by the number of Ly6G+ cells in every tumor node. Data points depict the mean CD3/Ly6G ratio derived from each tumor node in individual mice; NVehicle = 10, NGEM = 9, NCARI = 10, NGEM+CARI = 11 mice. (D) To obtain the CD3/Ly6G ratio per tumor node, the number of CD3+ cells was divided by the respective number of Ly6G+ cells in each tumor node. Data points depict individual tumor nodes; nVehicle = 386, nGEM = 276, nCARI = 301, nGEM+CARI = 398. Data and statistical comparison in D and E are represented as median ± 95% CI using Kruskal-Wallis statistical test with Dunn’s post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37643024), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Gr-1/Ly-6G by Immunohistochemistry

Detection of Mouse Gr-1/Ly-6G by Immunohistochemistry

Lymphocyte/neutrophil ratio increases upon NHE1 inhibition in tumor sections of KPfC mice.(A) H&E (left) and PAS-stained KPfC mouse tissue sections after vehicle and gemcitabine + cariporide (GEM+CARI) therapy. Cells of innate immunity, such as neutrophils (arrows), utilize glycogen and are thus PAS+ (purple), in contrast to, for example, lymphocytes. Scale bar: 50 μm. (B) Representative IHC images stained for Ly6G+ neutrophils (magenta, arrows on left image), CD3+ lymphocytes (yellow, arrows on the right image), and nuclei with DAPI (cyan). Scale bar: 50 μm. (C) CD3/Ly6G ratio was assessed by dividing the number of CD3+ cells by the number of Ly6G+ cells in every tumor node. Data points depict the mean CD3/Ly6G ratio derived from each tumor node in individual mice; NVehicle = 10, NGEM = 9, NCARI = 10, NGEM+CARI = 11 mice. (D) To obtain the CD3/Ly6G ratio per tumor node, the number of CD3+ cells was divided by the respective number of Ly6G+ cells in each tumor node. Data points depict individual tumor nodes; nVehicle = 386, nGEM = 276, nCARI = 301, nGEM+CARI = 398. Data and statistical comparison in D and E are represented as median ± 95% CI using Kruskal-Wallis statistical test with Dunn’s post hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37643024), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Ly-6G/Ly-6C (Gr-1) by Immunohistochemistry

Detection of Mouse Ly-6G/Ly-6C (Gr-1) by Immunohistochemistry

Lymphocyte/neutrophil ratio increases upon NHE1 inhibition in tumor sections of KPfC mice.(A) H&E (left) and PAS-stained KPfC mouse tissue sections after vehicle and gemcitabine + cariporide (GEM+CARI) therapy. Cells of innate immunity, such as neutrophils (arrows), utilize glycogen and are thus PAS+ (purple), in contrast to, for example, lymphocytes. Scale bar: 50 μm. (B) Representative IHC images stained for Ly6G+ neutrophils (magenta, arrows on left image), CD3+ lymphocytes (yellow, arrows on the right image), and nuclei with DAPI (cyan). Scale bar: 50 μm. (C) CD3/Ly6G ratio was assessed by dividing the number of CD3+ cells by the number of Ly6G+ cells in every tumor node. Data points depict the mean CD3/Ly6G ratio derived from each tumor node in individual mice; NVehicle = 10, NGEM = 9, NCARI = 10, NGEM+CARI = 11 mice. (D) To obtain the CD3/Ly6G ratio per tumor node, the number of CD3+ cells was divided by the respective number of Ly6G+ cells in each tumor node. Data points depict individual tumor nodes; nVehicle = 386, nGEM = 276, nCARI = 301, nGEM+CARI = 398. Data and statistical comparison in D and E are represented as median ± 95% CI using Kruskal-Wallis statistical test with Dunn’s post hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37643024), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Ly-6G/Ly-6C (Gr-1) by Immunohistochemistry

Detection of Ly-6G/Ly-6C (Gr-1) by Immunohistochemistry

Lymphocyte/neutrophil ratio increases upon NHE1 inhibition in tumor sections of KPfC mice.(A) H&E (left) and PAS-stained KPfC mouse tissue sections after vehicle and gemcitabine + cariporide (GEM+CARI) therapy. Cells of innate immunity, such as neutrophils (arrows), utilize glycogen and are thus PAS+ (purple), in contrast to, for example, lymphocytes. Scale bar: 50 μm. (B) Representative IHC images stained for Ly6G+ neutrophils (magenta, arrows on left image), CD3+ lymphocytes (yellow, arrows on the right image), and nuclei with DAPI (cyan). Scale bar: 50 μm. (C) CD3/Ly6G ratio was assessed by dividing the number of CD3+ cells by the number of Ly6G+ cells in every tumor node. Data points depict the mean CD3/Ly6G ratio derived from each tumor node in individual mice; NVehicle = 10, NGEM = 9, NCARI = 10, NGEM+CARI = 11 mice. (D) To obtain the CD3/Ly6G ratio per tumor node, the number of CD3+ cells was divided by the respective number of Ly6G+ cells in each tumor node. Data points depict individual tumor nodes; nVehicle = 386, nGEM = 276, nCARI = 301, nGEM+CARI = 398. Data and statistical comparison in D and E are represented as median ± 95% CI using Kruskal-Wallis statistical test with Dunn’s post hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37643024), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

Application
Recommended Usage

COMET

7 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse bone marrow cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

3-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen

Immunoprecipitation

Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.

Reviewed Applications

Read 8 reviews rated 4.6 using MAB1037 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

For research use only

Citations for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5)

Customer Reviews for Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) (8)

4.6 out of 5
8 Customer Ratings
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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 08/17/2021
    Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) MAB1037
  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Granulocytes
    Species: Mouse
    Verified Customer | Posted 06/02/2021
    Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) MAB1037
  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Pancreas tissue
    Species: Mouse
    Verified Customer | Posted 01/15/2021
    Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) MAB1037
  • Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lung cancer tissue
    Species: Mouse
    Verified Customer | Posted 04/18/2018
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Pancreatic cancer cells
    Species: pancreatic cancer cell line and Mouse
    Verified Customer | Posted 04/11/2018
  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: colon
    Species: Mouse
    Verified Customer | Posted 12/01/2017
    A. Normal mouse colon; B. DSS induced mouse colitis colon. The staining only work at 1:20 dilution instead of the recommended ~1:50 dilution. We tried 4 difference sources and this is the best one.
    Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) MAB1037
  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Melanie Ikeh
    Application: Flow Cytometry
    Sample Tested: Granulocytes
    Species: Mouse
    Verified Customer | Posted 07/17/2017
  • Mouse Ly-6G/Ly-6C (Gr-1) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Neutrophils
    Species: Mouse
    Verified Customer | Posted 05/30/2017

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