Mouse Mast Cell Protease-6/Mcpt6 Antibody

R&D Systems | Catalog # AF3736

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mast Cell Protease-6/Mcpt6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease-6/Mcpt6
Ala22-Ser276
Accession # P21845

Specificity

Detects Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TPS-1 and rhTPS-B2 is observed and less then 10% cross-reactivity with recombinant mouse MCP-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Detection of Mast Cell Protease-6/Mcpt6 by Immunocytochemistry/ Immunofluorescence

Detection of Mast Cell Protease-6/Mcpt6 by Immunocytochemistry/ Immunofluorescence

Stress and stress mediator effects on mMCP protein and mRNA expression in MCs. Data represent the mean values of the results from 5 animals (n = 5). ** = p ≤ 0.01; * = p ≤ 0.05. Immunohistomorphometric analysis was performed in 10 microscopic fields per animal on 14 µm thick skin cryosections for (a,d) mMCP4+ and mMCP6+ MCs (expressed in % of total number of FITC-Avidin+ MCs). FITC-Avidin is used as a specific MC marker in skin. (c,f) High- (black bar) and low-intensity (gray bar) staining of mMCP4+ and mMCP6+ MCs (sums up to % of mMCP+ MCs of the respective group given in (a,d)). (b,e) Representative photomicrographs of an immunohistochemical triple staining for mMCP4 and mMCP6 (red), MCs (FITC-labeled avidin, green), and cell nuclei (DAPI, blue), scale bar = 10 µm. Abbr.: AlD = atopic dermatitis-like allergic inflammation; anti-BDNF = antibody against BDNF; anti-NGF = antibody against NGF; BDNF = brain-derived neurotrophic factor; MC = mast cell; mMCP = murine mast cell protease; NGF = nerve growth factor; NiS = noise induced stress. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/11/5738), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (0.5 µg/mL, Catalog # 3736‑SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 3 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

References

  1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535 .
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.

Alternate Names

Mast Cell Protease6, Mcpt6

Entrez Gene IDs

7177 (Human); 17229 (Mouse); 29268 (Rat)

Gene Symbol

TPSAB1

UniProt

P21845

Additional Mast Cell Protease-6/Mcpt6 Products

Product Documents for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Mast Cell Protease-6/Mcpt6 Antibody

For research use only

Citations for Mouse Mast Cell Protease-6/Mcpt6 Antibody

Customer Reviews for Mouse Mast Cell Protease-6/Mcpt6 Antibody

There are currently no reviews for this product. Be the first to review Mouse Mast Cell Protease-6/Mcpt6 Antibody and earn rewards!

Have you used Mouse Mast Cell Protease-6/Mcpt6 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies