MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56‑58 kDa member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2 kDa ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417 amino acids (aa) in length. It contains an RGD motif and ASARM domain (aa 416‑433). In addition to its a 57 kDa form, 70 kDa, 42‑45 kDa and 30‑35 kDa forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa 147‑433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala17-Asp433
Accession # AAK70342
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse MEPE/OF45 Antibody
Detection of Mouse MEPE/OF45 by Western Blot.
Western blot shows lysates of ST-2 mouse bone marrow-derived stromal cell line and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MEPE/OF45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEPE/OF45 at approximately 58 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse MEPE/OF45 Antibody
Western Blot
Sample: ST‑2 mouse bone marrow-derived stromal cell line and mouse embryo tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MEPE/OF45
Long Name
Alternate Names
Gene Symbol
UniProt
Additional MEPE/OF45 Products
Product Documents for Mouse MEPE/OF45 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse MEPE/OF45 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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