Mouse Mer Antibody (108921)

Mer in J774A.1 Mouse Cell Line.

Mer was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB591) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse Mer by Western Blot

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mer by Western Blot

Expression of TAM RTKs, Gas6 and Protein S.(A) Western blot analyses of the liver lysates for the examination of TAM RTKs, Gas6 and Protein S. (B) Expression of TAM RTKs, Gas6 and Protein S in isolated liver cells: parenchymal cells (PCs), Kupffer cells(KCs) and sinusoidal endothelial cells (SECs). The primary cells were subjected to Western blotting. (C) Immunohistochemistry for the detection of TAM RTKs, Gas6 and Protein S. Arrowheads indicate PCs, and arrows indicate spindle-shaped sinusoidal cells corresponding to KCs and SECs. In negative controls (islets), sections were incubated with primary antibodies pre-incubated with an excess of blocking peptide. The livers of 15-week-old WT mice were used for the protein analyses. The images are representatives of at least three experiments. Scale bar = 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23799121), licensed under a CC-BY license. Not internally tested by R&D Systems.