Mouse MFRP Antibody [Biotin]

R&D Systems | Catalog # BAF3445

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all MFRP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Gln91-Pro584
Accession # Q8K480

Specificity

Detects mouse MFRP in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rmFrizzled-8, and rmFrizzled-10 is observed, 5% cross-reactivity with rhFrizzled-5 is observed, and 1% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse MFRP Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse MFRP

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MFRP

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Long Name

Membrane Frizzled-related Protein

Alternate Names

MCOP5, membrane frizzled-related protein, Membrane-type frizzled-related protein, MGC32938, NNO2, RD6

Entrez Gene IDs

83552 (Human); 259172 (Mouse)

Gene Symbol

MFRP

UniProt

Q8K480

Additional MFRP Products

Product Documents for Mouse MFRP Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MFRP Antibody [Biotin]

For research use only

Related Research Areas

Wnt Family
Wnt Signaling Modulators

Citations for Mouse MFRP Antibody [Biotin]

Customer Reviews for Mouse MFRP Antibody [Biotin]

There are currently no reviews for this product. Be the first to review Mouse MFRP Antibody [Biotin] and earn rewards!

Have you used Mouse MFRP Antibody [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies