Mouse Peptidase Inhibitor 16/PI16 Antibody

R&D Systems | Catalog # AF4929

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Peptidase Inhibitor 16/PI16 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Peptidase Inhibitor 16/PI16
Leu21-Glu464
Accession # Q9ET66

Specificity

Detects mouse Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Peptidase Inhibitor 16/PI16 Antibody

Peptidase Inhibitor 16/PI16 in Mouse Pancreas.

Peptidase Inhibitor 16/PI16 in Mouse Pancreas.

Mouse pancreas cryosections stained with CD324 (green) and PI16 (white). Image from a verified customer review.
Detection of Peptidase Inhibitor 16/PI16 by Immunohistochemistry

Detection of Peptidase Inhibitor 16/PI16 by Immunohistochemistry

Foxs1 knockdown in fibroblasts inhibits tumor growth.(A) Photographs of excised tumors from shNC and shFoxs1 mice. (B) Tumor volume over time. Data represent mean ± SD; n = 8. Statistical significance was assessed at each time point using an unpaired two-tailed Student’s t test. Significant exact P values were: Day 10: shNC vs. shFoxs1, P = 0.0008; Day 12: shNC vs. shFoxs1, P = 1.49 × 10−4; Day 14: shNC vs. shFoxs1, P = 5.66 × 10−6; Day 16: shNC vs. shFoxs1, P = 5.13 × 10−6. *P < 0.05. (C) Quantification of tumor weight. Data represent mean ± SD; n = 8. Statistical significance was assessed using an unpaired two-tailed Student’s t test; shNC vs. shFoxs1, P = 6.82 × 10−7; *P < 0.05. (D, E) IF staining for CD34, PI16, and alpha -SMA/COL1A1 in tumor tissues from the two mouse groups (D), with quantification (E). Scale Bars:100 μm. Data are mean ± SD; n = 5. Statistical significance was assessed using an unpaired two-tailed Student’s t test; Significant exact P values were: CD34, shNC vs. shFoxs1, P = 0.0002; PI16, shNC vs. shFoxs1, P = 0.0011; alpha -SMA, shNC vs. shFoxs1, P = 0.0001; *P < 0.05. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1038/s44321-026-00476-8), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Peptidase Inhibitor 16/PI16 by Western Blot

Detection of Peptidase Inhibitor 16/PI16 by Western Blot

CD34+ PI16+ SPC subpopulation and the pro-inflammatory effect of CD34+ PI16- cell subpopulation. (A) UMAP plots showing the major cell clusters of CD34+ cells in the normal thoracic aortas and grafted arteries (n = 43178 cells). (B) Bar chart showing the proportion of identified cell clusters in the normal thoracic aortas and grafted arteries. (C) Volcano plots showing the up-regulated gene for clusters 0 and 3. (D) Feature plots showing CD34 and PI16 expression in different clusters. (E) Gene Ontology analysis of top enriched functions in cluster 0. (F) Gene Ontology analysis of top enriched functions in cluster 3. (G) Analysis of the correlation between each cluster and the chemokine family. (H) Pseudotime trajectories showing CD34high and PI16high cells differentiated into endothelial cells and fibroblasts in the normal thoracic aortas and grafted arteries. (I) Trajectory plot showing the pseudotime representing trajectories of Mef2c+ and PDGFR-alpha + cells differentiation and the expression of 2 markers (CD34/Ly6a) in the endothelial cells. (J) Representative western blot images of these markers (PDGFR-alpha /Postn/CD34/PDGFR-beta /Vimentin/PI16) from CD34+ PI16+ cells treated with PBS and CTGF 50ug/L for 48 h. n = 6 per group. (K) Quantification analysis of these markers in (J). Data were presented as the mean ± SEM and analyzed by using an unpaired two-tailed Student's t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (L) Cartoon showing that CD34+ PI16- cells, derived from CD34+ PI16+ cells, have a chemotactic effect on SMCs. Image collected and cropped by CiteAb from the following open publication (https://www.thno.org/v15p2523.htm), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Peptidase Inhibitor 16/PI16 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Peptidase Inhibitor 16/PI16

Reviewed Applications

Read 1 review rated 4 using AF4929 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Peptidase Inhibitor 16/PI16

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS-PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Alternate Names

CRISP9, MSMBBP, PI16, PSPBP

Entrez Gene IDs

221476 (Human); 74116 (Mouse); 294312 (Rat)

Gene Symbol

PI16

UniProt

Q9ET66

Additional Peptidase Inhibitor 16/PI16 Products

Product Documents for Mouse Peptidase Inhibitor 16/PI16 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Peptidase Inhibitor 16/PI16 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Mouse Peptidase Inhibitor 16/PI16 Antibody

Customer Reviews for Mouse Peptidase Inhibitor 16/PI16 Antibody (1)

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  • Mouse Peptidase Inhibitor 16/PI16 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult pancreas
    Species: Mouse
    Verified Customer | Posted 10/14/2025
    Mouse pancreas cryosections stained with CD324 (green) and PI16 (white)
    Mouse Peptidase Inhibitor 16/PI16 Antibody AF4929
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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