Mouse Rae-1 alpha/beta/gamma Antibody (199215)

R&D Systems | Catalog # MAB1758

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 199215
View all Rae-1 alpha/beta/gamma Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1 delta

Specificity

Detects mouse Rae‑1 alpha / beta / gamma. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha, beta and gamma. It does not recognize Rae-1 delta or epsilon.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Rae-1 alpha/beta/gamma Antibody (199215)

Detection of Rae-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 alpha/beta/gamma Monoclonal Antibody (Catalog # MAB1758, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Rae-1 alpha/beta/gamma Antibody (199215)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # 139-NK) to immobilized Recombinant Mouse Rae-1 beta Fc Chimera (Catalog # 1198-RA) coated at 1 µg/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding. This antibody also blocks the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha and Rae-1 gamma.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rae-1 alpha/beta/gamma

The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.

Long Name

Retinoic Acid Early Transcript 1

Entrez Gene IDs

8480 (Human); 66679 (Mouse); 362281 (Rat)

Gene Symbol

RAE1

Additional Rae-1 alpha/beta/gamma Products

Product Documents for Mouse Rae-1 alpha/beta/gamma Antibody (199215)

Certificate of Analysis

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Product Specific Notices for Mouse Rae-1 alpha/beta/gamma Antibody (199215)

For research use only

Citations for Mouse Rae-1 alpha/beta/gamma Antibody (199215)

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