Butyrylcholinesterase/BCHE Antibody

Detection of Mouse Butyrylcholinesterase/BCHE by Western Blot.

Western blot shows lysates of mouse heart tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Polyclonal Antibody (Catalog # AF9024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Butyrylcholinesterase/BCHE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Butyrylcholinesterase/BCHE in Mouse Brain.

Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Butyrylcholinesterase/BCHE by Immunohistochemistry

Conservation of ACP and ACV markers across postnatal development.a, Immunostaining of ACP neurons in the rostral Amb of a neonatal (postnatal day 2) mouse. ACP neurons stained positive for calbindin (red) and stained weakly positive for BChE (cyan). b, Immunostaining of neonatal ACV neurons in the caudal Amb of a postnatal day 2 mouse. ACV neurons stained positive for BChE and negative for calbindin. Bars, 20 μm. c, Map of ACP and ACV neurons in neonatal Amb. Sagittal schematic view showing overlay of soma of all neurons (circles) across all sections spanning a single neonatal (postnatal day 2) Amb that was stained for ACP marker calbindin (dark blue circles) and ACV marker BChE (light blue circles). d, Quantification of absolute numbers of ACP neurons (calbindin+, dark blue) and ACV neurons (BChE+, light blue) per Amb in neonatal mice (mean ± s.d., n = 3 mice). e, Immunostaining of ACP neurons in the rostral Amb of an adult (postnatal day 60) mouse. ACP neurons stained positive for calbindin (red) and stained weakly positive for BChE (cyan). f, Immunostaining of adult ACV neurons in the caudal Amb of a postnatal day 60 mouse. ACV neurons stained positive for BChE and negative for calbindin. Bars, 20 μm. g, Map of ACP and ACV neurons in adult Amb. Sagittal schematic view showing overlay of soma of all neurons (circles) across all sections spanning a single adult (postnatal day 60) Amb that was stained for calbindin (dark blue) and BChE (light blue). Note similarity in marker expression and cell type distribution in neonatal and adult Amb. h, Quantification of absolute numbers of ACP neurons (calbindin+, dark blue) and ACV neurons (BChE+, light blue) per Amb in adult mice (mean ± s.d., n = 3 mice). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41586-022-04760-8), licensed under a CC-BY license. Not internally tested by R&D Systems.