SIGNR3/CD209d Antibody (728804)

R&D Systems | Catalog # MAB7220

R&D Systems

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 728804
View all SIGNR3/CD209d Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
Glu82-Lys237
Accession # Q91ZW8

Specificity

Detects mouse and rat SIGNR3/CD209d in Western blots. In Western blots, 100% cross-reactivity with recombinant mouse (rm) SIGNR1, 50% cross-reactivity with rmSIGNR7, and no cross-reactivity with rmSIGNR4 is observed. In direct ELISAs, no cross-reactivity with recombinant human (rh) DC-SIGN, rhDC-SIGNR, rmSIGNR1, rmSIGNR4, or rmSIGNR7 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for SIGNR3/CD209d Antibody (728804)

Detection of Mouse and Rat SIGNR3/CD209d antibody by Western Blot.

Detection of Mouse and Rat SIGNR3/CD209d by Western Blot.

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat SIGNR3/CD209d Monoclonal Antibody (Catalog # MAB7220) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for SIGNR3/CD209d at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for SIGNR3/CD209d Antibody (728804)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIGNR3/CD209d

SIGNR3 (designated CD209d) is a 237 amino acid (aa), ~34 kDa type II transmembrane glycoprotein that is one of several mouse orthologs of human DC-SIGN (CD209). SIGNR3 is a calcium-binding C-type lectin that is expressed on myeloid mononuclear cells and a subpopulation of dendritic cells in the dermis, blood, and most lymphoid organs. Like human DC-SIGN, SIGNR3 recognizes high-mannose and fucose-terminated glycans and protects against pathogens such as Candida albicans and Mycobacterium tuberculosis. Within the region used as an immunogen, mouse and rat SIGNR3 share 91% aa sequence identity. A 207 aa isoform lacks aa 45-74, which includes the transmembrane domain.

Long Name

DC-SIGN-related Protein 3

Alternate Names

CD209d

Entrez Gene IDs

170779 (Mouse); 363856 (Rat)

Gene Symbol

CD209D

UniProt

Q91ZW8 (Mouse)

Additional SIGNR3/CD209d Products

Product Documents for SIGNR3/CD209d Antibody (728804)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SIGNR3/CD209d Antibody (728804)

For research use only

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