Mouse Resistin Antibody (175322)

R&D Systems | Catalog # MAB1069

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 175322
View all Resistin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Resistin
Ser21-Ser114
Accession # Q99P87

Specificity

Detects mouse Resistin in ELISAs and Western blots. Does not cross-react with recombinant mouse RELM alpha.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Resistin Antibody (175322)

Mouse Resistin ELISA Standard Curve

Mouse Resistin ELISA Standard Curve

Recombinant Mouse Resistin (Catalog # 1069-RN) was serially diluted and captured by Rat Anti-Mouse Resistin Monoclonal Antibody (Catalog # MAB1069) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Resistin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1069) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse Resistin Antibody (175322)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Resistin (Catalog # 1069-RN)
under non-reducing conditions only

Mouse Resistin Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Resistin Biotinylated Antibody (Catalog # BAF1069)
  • Standard: Recombinant Mouse Resistin Protein (Catalog # 1069-RN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Resistin

Resistin (for resistance to insulin), also known as FIZZ3 (found in inflammatory zone 3), is a secreted, cysteine-rich, adipocyte-derived protein. It contributes to insulin resistance associated with type 2 diabetes mellitus and may be the hormone that links obesity to diabetes.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Gene Symbol

RETN

UniProt

Q99P87

Additional Resistin Products

Product Documents for Mouse Resistin Antibody (175322)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Resistin Antibody (175322)

For research use only

Related Research Areas

Glucose Homeostasis
Obesity

Citations for Mouse Resistin Antibody (175322)

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways
Adipokines & Insulin Signaling Pathway Thumbnail