Mouse S100A8 Antibody (335806)

R&D Systems | Catalog # MAB3059

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 335806
View all S100A8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse S100A8
Met1-Glu89 (predicted)
Accession # P27005

Specificity

Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse S100A8 Antibody (335806)

Detection of S100A8 Rat antibody by Western Blot.

Detection of S100A8 Rat by Western Blot.

Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A8 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC).

S100A8 in NIH-3T3 Mouse Cell Line.

S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of S100A8 by Immunohistochemistry

Detection of S100A8 by Immunohistochemistry

IACS-70654 suppressed the growth of neutrophil-enriched preclinical mouse models of TNBC.(A) Representative images of H&E and immunostaining of all TNBC preclinical mouse models used. F4/80 is a macrophage marker, and S100A8 is a neutrophil marker. Scale bars: 50 μm. (B) Flow cytometry analysis of TANs and TAMs in all TNBC preclinical mouse models used (T6, n = 6; all other models, n = 5). (C) The treatment scheme of IACS-70654 in a 7-day experiment. When the average tumor volume reached 80–250 mm3, the mice were randomized to initiate treatment. Vehicle or IACS-70654 at 3.75 mg/kg was administered orally on a 3-on/2-off schedule. On day 7, mice were euthanized 2 hours after receiving the last treatment. (D) Tumor growth curves of all TNBC models treated with IACS-70654 for 7 days (2208L, n = 6; all other models, n = 5). Two-way ANOVA and Šidák’s multiple-comparison test were used. (E) Left: Representative images of immunostaining of BrdU in 2208L tumor sections treated with vehicle or IACS-70654 for 7 days. Scale bar: 50 μm. Right: Quantification of BrdU staining (n = 15). A 2-tailed, unpaired Student’s t test was used. For B, D, and E, data are presented as mean ± SD. *P < 0.05; ****P < 0.0001. The experiments were conducted 3 times for 2208L and twice for other models. Data from 1 representative experiment are shown. (F) Tumor growth curves (from 1 representative experiment) of 2208L tumors treated with IACS-70654 for 27 days. The experiment was conducted twice. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39287984), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of S100A8 by Immunohistochemistry

Detection of S100A8 by Immunohistochemistry

IACS-70654 reduced the growth of established 2208L lung metastases.(A) Violin plots showing RNA expression of representative genes that might be involved in migration and metastasis in 2208L tumor cells treated in vivo. Adjusted P < 0.01 for all genes. (B) Experimental design for studying the effects of IACS-70654 on established lung metastases. Dissociated 2208L tumor cells (100,000) were injected into the tail vein of each mouse. The mice were treated for 23 days. One mouse from the vehicle group had to be euthanized on day 22 due to poor body condition. (C) Representative images from H&E staining of lungs with 2208L metastases from mice after treatment. Scale bars: 5 mm. (D) Quantifications of lung metastases after treatment. Serial sectioning was performed to collect a total of 10 sections for each sample (vehicle, n = 40; IACS-70654, n = 50). Metastases with sizes of <1 mm, 1–3 mm, 3–5 mm, and >5 mm were assigned scores of 1, 2, 3, and 4, respectively. A 2-tailed, unpaired Student’s t test was used. Data are presented as mean ± SD. (E) Left: Representative images of S100A8 immunostaining on 2208L lung metastasis sections. Scale bar: 50 μm. Right: Quantification of S100A8 staining (n = 12). A 2-tailed, unpaired Student’s t test was used. ***P < 0.001; ****P < 0.0001. Data are presented as mean ± SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39287984), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse S100A8 Antibody (335806)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line

Western Blot

2 µg/mL
Sample: Mouse leukocyte

Reviewed Applications

Read 1 review rated 5 using MAB3059 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Entrez Gene IDs

6279 (Human); 20201 (Mouse)

Gene Symbol

S100A8

UniProt

P27005

Additional S100A8 Products

Product Documents for Mouse S100A8 Antibody (335806)

Certificate of Analysis

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Product Specific Notices for Mouse S100A8 Antibody (335806)

For research use only

Citations for Mouse S100A8 Antibody (335806)

Customer Reviews for Mouse S100A8 Antibody (335806) (1)

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  • Mouse S100A8 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Pulmonary Artery Smooth Muscle Cells and Artery Smooth Muscle Cells
    Species: Mouse
    Verified Customer | Posted 11/02/2021
    Mouse S100A8 Antibody (335806) MAB3059

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Protocols

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