This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Leu27-Gly119
Accession # CAA28351
Specificity
Detects Sca‑1/Ly6 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Sca-1/Ly6 Antibody [Biotin]
Sca‑1/Ly6 in Mouse Splenocytes.
Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse Sca-1/Ly6 Antibody [Biotin]
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Sca‑1/Ly6
Sample: Recombinant Mouse Sca‑1/Ly6
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Sca-1/Ly6
References
- Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
- Spangrude, G.I. et al. (1988) Science 241:58.
- Spangrude, G.I. et al. (1993) Blood 82:3327.
- Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
- Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
- Yamamoto, Y. et al. (1996) Blood 88:445.
- Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
- Welm, B.E. et al. (2002) Dev. Biol. 245:42.
- Codias, E.K. et al. (1990) J. Immunol. 144:2197.
- Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
- Codias, E.K. et al. (1990) J. Immunol. 145:1407.
- Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Alternate Names
Ly-6, Ly6, Sca1
Entrez Gene IDs
110454 (Mouse)
Gene Symbol
LY6A
UniProt
Additional Sca-1/Ly6 Products
Product Documents for Mouse Sca-1/Ly6 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Sca-1/Ly6 Antibody [Biotin]
For research use only
Customer Reviews for Mouse Sca-1/Ly6 Antibody [Biotin]
There are currently no reviews for this product. Be the first to review Mouse Sca-1/Ly6 Antibody [Biotin] and earn rewards!
Have you used Mouse Sca-1/Ly6 Antibody [Biotin]?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...