Mouse Shrew-1/AJAP1 Antibody

R&D Systems | Catalog # AF7970

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation, Binding Assay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Shrew-1/AJAP1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Shrew-1/AJAP1
His157-His283
Accession # A2ALI5

Specificity

Detects mouse Shrew-1/AJAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human AJAP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Shrew-1/AJAP1 Antibody

Detection of Mouse Shrew-1/AJAP1 antibody by Western Blot.

Detection of Mouse Shrew-1/AJAP1 by Western Blot.

Western blot shows lysates of mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Shrew-1/AJAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7970) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Shrew-1/ AJAP1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Shrew-1/AJAP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse brain (cortex) tissue, mouse brain (thalamus/hypothalamus) tissue, and mouse pancreas tissue

Reviewed Applications

Read 2 reviews rated 5 using AF7970 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Shrew-1/AJAP1

AJAP-1 (Adherens Junction Associated Protein 1; also Shrew-1 and Gm573) is a 44-47 kDa transmembrane protein found on the basolateral surface of epithelium. It is presumably expressed by multiple types of epithelium, and participates in cell-cell adhesion. Zonula adherens are structures that participate in the generation of cell contact adherens junctions. Such junctions, or complexes, are key to the maintenance of epithelial cell polarity and the maintenance of cell-to-cell contact. Central to this complex is E-cadherin, a transmembrane molecule that makes crucial contacts with cytoskeletal actin via catenins. AJAP-1 is an accompaning transmembrane molecule that interacts with E-cadherin and appears to promote the internalization of E-cadherin upon growth factor stimulation. This facilitates the dissolution of adherens junctions with an increase in cell motility. Notably, AJAP-1 is also known to complex with CD147 in nonpolar, invasive cells, suggesting that AJAP-1 may play a more complex role in cell migration. Mouse AJAP-1 is a 412 amino acid (aa) type III (no signal sequence) transmembrane protein. It contains a 284 aa extracellular region (aa 1-284) plus a 106 aa cytoplasmic domain (aa 306-412). Over aa 1-283, mouse AJAP-1 shares 95% and 75% aa sequence identity with rat and human AJAP-1, respectively; over aa 158-283, mouse AJAP-1 sequence identity changes little, showing 95% and 78% equality with rat and human AJAP-1, respectively.

Long Name

Transmembrane Protein Shrew-1/Adherens Junction Associated Protein 1

Alternate Names

AJAP1, Gm573, MOT8, Shrew1

Entrez Gene IDs

55966 (Human); 230959 (Mouse)

Gene Symbol

AJAP1

UniProt

A2ALI5

Additional Shrew-1/AJAP1 Products

Product Documents for Mouse Shrew-1/AJAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Shrew-1/AJAP1 Antibody

For research use only

Citations for Mouse Shrew-1/AJAP1 Antibody

Customer Reviews for Mouse Shrew-1/AJAP1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Mouse Shrew-1/AJAP1 Antibody
    Name: Elizabeth Allen
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HEK293T human embryonic kidney cell line and schwann cells
    Species: Human
    Verified Customer | Posted 09/06/2021
    Cells were transfected with plasmids that express mouse Ajap1-C-EGFP. We diluted the Ajap1 antibody 1:500 in blocking buffer (10% donkey serum, 0.1% Tween 20 in PBS), and incubated overnight at 4ºC. We used a donkey anti-sheep-555 from abcam at 1:500 (same blocking buffer as above) with DAPI to stain nuclei.
    Mouse Shrew-1/AJAP1 Antibody AF7970
  • Mouse Shrew-1/AJAP1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 01/17/2018
    Mouse Shrew-1/AJAP1 Antibody AF7970

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