Mouse Siglec-1/CD169 Antibody (645608)

R&D Systems | Catalog # MAB5610

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 645608
View all Siglec-1/CD169 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
Thr20-Leu1639 (predicted)
Accession # Q62230

Specificity

Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec-1, -5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Siglec-1/CD169 Antibody (645608)

Detection of Mouse Siglec-1/CD169 antibody by Western Blot.

Detection of Mouse Siglec‑1/CD169 by Western Blot.

Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 1 µg/mL of Mouse Siglec-1/CD169 Monoclonal Antibody (Catalog # MAB5610) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Siglec-1/CD169 at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Detection of Siglec‑1/CD169 in RAW264 cells by Flow Cytometry.

RAW264 cells stimulated with 1 ug/mL LPS overnight were stained with Rat Anti-Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Siglec-1/CD169 Antibody (645608)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight 

Western Blot

1 µg/mL
Sample: Mouse splenocytes under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB5610 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-1/CD169

Siglec-1, also known as sialoadhesin or CD169, is a 175‑185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human); 20612 (Mouse); 311426 (Rat)

Gene Symbol

SIGLEC1

UniProt

Q62230

Additional Siglec-1/CD169 Products

Product Documents for Mouse Siglec-1/CD169 Antibody (645608)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Siglec-1/CD169 Antibody (645608)

For research use only

Citations for Mouse Siglec-1/CD169 Antibody (645608)

Customer Reviews for Mouse Siglec-1/CD169 Antibody (645608) (2)

5 out of 5
2 Customer Ratings
5 Stars
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  • Mouse Siglec-1/CD169 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 01/24/2021
    RAW 264.7 cell lysates were prepared in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, pH 7.6, including protease inhibitors, 1 ug/ml leupeptin, 1 ug/ml aprotinin, and 1 mM phenylmethylsulfonyl fluoride).
    Mouse Siglec-1/CD169 Antibody (645608) MAB5610
  • Mouse Siglec-1/CD169 Antibody
    Name: Oonagh Paerewijck
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 02/02/2018
    rat anti-Siglec + goat-anti-rat
    Mouse Siglec-1/CD169 Antibody (645608) MAB5610

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